This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1p4o
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:1p4o.gif|left|200px]] | [[Image:1p4o.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_1p4o", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_1p4o| PDB=1p4o | SCENE= }} | |
| - | + | ||
| - | + | ||
| - | }} | + | |
'''Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.''' | '''Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.''' | ||
| Line 35: | Line 32: | ||
[[Category: Munshi, S.]] | [[Category: Munshi, S.]] | ||
[[Category: Waxman, L.]] | [[Category: Waxman, L.]] | ||
| - | [[Category: | + | [[Category: Igf-1r]] |
| - | [[Category: | + | [[Category: Kinase domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:40:55 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:40, 3 May 2008
Structure of Apo unactivated IGF-1R KInase domain at 1.5A resolution.
Contents |
Overview
The crystal structure of the wild-type unactivated kinase domain (IGFRK-0P) of insulin-like growth factor-1 receptor has been reported previously at 2.7 A resolution [Munshi et al. (2002), J. Biol. Chem. 277, 38797-38802]. In order to obtain a high-resolution structure, a number of variants of IGFRK-0P were prepared and screened for crystallization. A double mutant with E1067A and E1069A substitutions within the kinase-insert region resulted in crystals that diffracted to 1.5 A resolution. Overall, the structure of the mutant IGFRK-0P is similar to that of the wild-type IGFRK-0P structure, with the exception of the previously disordered kinase-insert region in the wild type having become fixed. In addition, amino-acid residues 947-952 at the N-terminus are well defined in the mutant structure. The monomeric protein structure is folded into two lobes connected by a hinge region, with the catalytic center situated at the interface of the two lobes. Two molecules of IGFRK-0P in the asymmetric unit are associated as a dimer and two different types of dimers with their ATP-binding clefts either facing towards or away from each other are observed. The current refined model consists of a dimer and 635 water molecules.
Disease
Known disease associated with this structure: Intrauterine and postnatal growth retardation OMIM:[147370]
About this Structure
1P4O is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of apo, unactivated insulin-like growth factor-1 receptor kinase at 1.5 A resolution., Munshi S, Hall DL, Kornienko M, Darke PL, Kuo LC, Acta Crystallogr D Biol Crystallogr. 2003 Oct;59(Pt 10):1725-30. Epub 2003, Sep 19. PMID:14501110 Page seeded by OCA on Sat May 3 04:40:55 2008
