This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1qoi
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1qoi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1qoi, resolution 2.00Å" /> '''U4/U6 SNRNP-SPECIFI...)
Next diff →
Revision as of 16:48, 12 November 2007
|
U4/U6 SNRNP-SPECIFIC CYCLOPHILIN SNUCYP-20
Contents |
Overview
The cyclophilin SnuCyp-20 is a specific component of the human U4/U6 small, nuclear ribonucleoprotein particle involved in the nuclear splicing of, pre-mRNA. It stably associates with the U4/U6-60kD and -90kD proteins, the, human orthologues of the Saccharomyces cerevisiae Prp4 and Prp3 splicing, factors. We have determined the crystal structure of SnuCyp-20 at 2.0-A, resolution by molecular replacement. The structure of SnuCyp-20 closely, resembles that of human cyclophilin A (hCypA). In particular, the, catalytic centers of SnuCyp-20 and hCypA superimpose perfectly, which is, reflected by the observed peptidyl-prolyl-cis/trans-isomerase activity of, SnuCyp-20. The surface properties of both proteins, however, differ, significantly. Apart from seven additional amino-terminal residues, the, insertion of five amino acids in the loop alpha1-beta3 and of one amino, acid in the loop alpha2-beta8 changes the conformations of both loops. The, enlarged loop alpha1-beta3 is involved in the formation of a wide cleft, with predominantly hydrophobic character. We propose that this enlarged, loop is required for the interaction with the U4/U6-60kD protein.
Disease
Known diseases associated with this structure: Cardiomyopathy, dilated OMIM:[605906], Cardiomyopathy, dilated, with left ventricular noncompaction OMIM:[605906], Myopathy, myofibrillar, ZASP-related OMIM:[605906]
About this Structure
1QOI is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structure of the human U4/U6 small nuclear ribonucleoprotein particle-specific SnuCyp-20, a nuclear cyclophilin., Reidt U, Reuter K, Achsel T, Ingelfinger D, Luhrmann R, Ficner R, J Biol Chem. 2000 Mar 17;275(11):7439-42. PMID:10713041
Page seeded by OCA on Mon Nov 12 18:55:10 2007
Categories: Homo sapiens | Single protein | Achsel, T. | Ficner, R. | Ingelfinger, D. | Luehrmann, R. | Reidt, U. | Reuter, K. | Cyclophilin | Snrnp | Snucyp-20 | Spliceosomal
