3zpv

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain

Structural highlights

3zpv is a 36 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.68Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PYGO_DROME Involved in signal transduction through the Wnt pathway.

Publication Abstract from PubMed

Pygo proteins promote Armadillo- and beta-catenin-dependent transcription, by relieving Groucho-dependent repression of Wnt targets. Their PHD fingers bind histone H3 tail methylated at lysine 4, and to the HD1 domain of their Legless/BCL9 cofactors, linking Pygo to Armadillo/beta-catenin. Intriguingly, fly Pygo orthologs exhibit a tryptophan > phenylalanine substitution in their histone pocket-divider which reduces their affinity for histones. Here, we use X-ray crystallography and NMR, to discover a conspicuous groove bordering this phenylalanine in the Drosophila PHD-HD1 complex-a semi-aromatic cage recognizing asymmetrically methylated arginine 2 (R2me2a), a chromatin mark of silenced genes. Our structural model of the ternary complex reveals a distinct mode of dimethylarginine recognition, involving a polar interaction between R2me2a and its groove, the structural integrity of which is crucial for normal tissue patterning. Notably, humanized fly Pygo derepresses Notch targets, implying an inherent Notch-related function of classical Pygo orthologs, disabled in fly Pygo, which thus appears dedicated to Wnt signaling.

Evolutionary Adaptation of the Fly Pygo PHD Finger toward Recognizing Histone H3 Tail Methylated at Arginine 2.,Miller TC, Mieszczanek J, Sanchez-Barrena MJ, Rutherford TJ, Fiedler M, Bienz M Structure. 2013 Oct 30. pii: S0969-2126(13)00364-X. doi:, 10.1016/j.str.2013.09.013. PMID:24183574^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Miller TC, Mieszczanek J, Sanchez-Barrena MJ, Rutherford TJ, Fiedler M, Bienz M. Evolutionary Adaptation of the Fly Pygo PHD Finger toward Recognizing Histone H3 Tail Methylated at Arginine 2. Structure. 2013 Oct 30. pii: S0969-2126(13)00364-X. doi:, 10.1016/j.str.2013.09.013. PMID:24183574 doi:http://dx.doi.org/10.1016/j.str.2013.09.013

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3zpv"

@@ Line 3: / Line 3: @@
 <StructureSection load='3zpv' size='340' side='right'caption='[[3zpv]], [[Resolution|resolution]] 2.68&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3zpv]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZPV FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3zpv]] is a 36 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZPV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZPV FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.68&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zpv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zpv OCA], [https://pdbe.org/3zpv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zpv RCSB], [https://www.ebi.ac.uk/pdbsum/3zpv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zpv ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/BCL9_DROME BCL9_DROME]] Involved in signal transduction through the Wnt pathway. [[https://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME]] Involved in signal transduction through the Wnt pathway.
+[https://www.uniprot.org/uniprot/PYGO_DROME PYGO_DROME] Involved in signal transduction through the Wnt pathway.
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Drome]]
+[[Category: Drosophila melanogaster]]
 [[Category: Large Structures]]
-[[Category: Bienz, M]]
+[[Category: Bienz M]]
-[[Category: Fiedler, M]]
+[[Category: Fiedler M]]
-[[Category: Mieszczanek, J]]
+[[Category: Mieszczanek J]]
-[[Category: Miller, T C.R]]
+[[Category: Miller TCR]]
-[[Category: Rutherford, T J]]
+[[Category: Rutherford TJ]]
-[[Category: Sanchez-Barrena, M J]]
+[[Category: Sanchez-Barrena MJ]]
-[[Category: Histone h3 tail binding]]
-[[Category: Transcription]]
-[[Category: Wnt signaling pathway]]
-[[Category: Zn finger]]

3zpv

From Proteopedia

Current revision

Crystal structure of Drosophila Pygo PHD finger in complex with Legless HD1 domain

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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