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| | <StructureSection load='3zqz' size='340' side='right'caption='[[3zqz]], [[Resolution|resolution]] 2.35Å' scene=''> | | <StructureSection load='3zqz' size='340' side='right'caption='[[3zqz]], [[Resolution|resolution]] 2.35Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[3zqz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drome Drome]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQZ FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[3zqz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3ZQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3ZQZ FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SLC:SELENO-CAPTOPRIL'>SLC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.35Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[2x94|2x94]], [[1j38|1j38]], [[2x8z|2x8z]], [[1j36|1j36]], [[2xhm|2xhm]], [[2x8y|2x8y]], [[2x91|2x91]], [[2x95|2x95]], [[2x92|2x92]], [[2x96|2x96]], [[2x93|2x93]], [[2x97|2x97]], [[2x90|2x90]], [[1j37|1j37]]</div></td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SLC:SELENO-CAPTOPRIL'>SLC</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Peptidyl-dipeptidase_A Peptidyl-dipeptidase A], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.15.1 3.4.15.1] </span></td></tr>
| + | |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqz OCA], [https://pdbe.org/3zqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqz RCSB], [https://www.ebi.ac.uk/pdbsum/3zqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqz ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3zqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3zqz OCA], [https://pdbe.org/3zqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3zqz RCSB], [https://www.ebi.ac.uk/pdbsum/3zqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3zqz ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ACE_DROME ACE_DROME]] May be involved in the specific maturation or degradation of a number of bioactive peptides. May play a role in the contractions of the heart, gut and testes, and in spermatid differentiation.<ref>PMID:12591244</ref>
| + | [https://www.uniprot.org/uniprot/ACE_DROME ACE_DROME] May be involved in the specific maturation or degradation of a number of bioactive peptides. May play a role in the contractions of the heart, gut and testes, and in spermatid differentiation.<ref>PMID:12591244</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Drome]] | + | [[Category: Drosophila melanogaster]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Peptidyl-dipeptidase A]]
| + | [[Category: Acharya KR]] |
| - | [[Category: Acharya, K R]] | + | [[Category: Akif M]] |
| - | [[Category: Akif, M]] | + | [[Category: Bhuyan BJ]] |
| - | [[Category: Bhuyan, B J]] | + | [[Category: Isaac RE]] |
| - | [[Category: Isaac, R E]] | + | [[Category: Masuyer G]] |
| - | [[Category: Masuyer, G]] | + | [[Category: Mugesh G]] |
| - | [[Category: Mugesh, G]] | + | [[Category: Schwager SLU]] |
| - | [[Category: Schwager, S L.U]] | + | [[Category: Sturrock ED]] |
| - | [[Category: Sturrock, E D]] | + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Inhibitor design]]
| + | |
| Structural highlights
3zqz is a 1 chain structure with sequence from Drosophila melanogaster. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
| | Method: | X-ray diffraction, Resolution 2.35Å |
| Ligands: | , , , , |
| Resources: | FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT |
Function
ACE_DROME May be involved in the specific maturation or degradation of a number of bioactive peptides. May play a role in the contractions of the heart, gut and testes, and in spermatid differentiation.[1]
Publication Abstract from PubMed
Human somatic angiotensin I-converting enzyme (ACE), a zinc-dependent dipeptidyl carboxypeptidase, is central to the regulation of the renin-angiotensin aldosterone system. It is a well-known target for combating hypertension and related cardiovascular diseases. In a recent study by Bhuyan and Mugesh [Org. Biomol. Chem. (2011) 9, 1356-1365], it was shown that the selenium analogues of captopril (a well-known clinical inhibitor of ACE) not only inhibit ACE, but also protect against peroxynitrite-mediated nitration of peptides and proteins. Here, we report the crystal structures of human testis ACE (tACE) and a homologue of ACE, known as AnCE, from Drosophila melanogaster in complex with the most promising selenium analogue of captopril (SeCap) determined at 2.4 and 2.35 A resolution, respectively. The inhibitor binds at the active site of tACE and AnCE in an analogous fashion to that observed for captopril and provide the first examples of a protein-selenolate interaction. These new structures of tACE-SeCap and AnCE-SeCap inhibitor complexes presented here provide important information for further exploration of zinc coordinating selenium-based ACE inhibitor pharmacophores with significant antioxidant activity. Database Structural data for the two SeCap complexes with ACE and AnCE have been deposited with the RCSB Protein Data Bank under the codes 2YDM and 3ZQZ, respectively.
Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril.,Akif M, Masuyer G, Schwager SL, Bhuyan BJ, Mugesh G, Isaac RE, Sturrock ED, Acharya KR FEBS J. 2011 Aug 2. doi: 10.1111/j.1742-4658.2011.08276.x. PMID:21810173[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Hurst D, Rylett CM, Isaac RE, Shirras AD. The drosophila angiotensin-converting enzyme homologue Ance is required for spermiogenesis. Dev Biol. 2003 Feb 15;254(2):238-47. PMID:12591244
- ↑ Akif M, Masuyer G, Schwager SL, Bhuyan BJ, Mugesh G, Isaac RE, Sturrock ED, Acharya KR. Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril. FEBS J. 2011 Aug 2. doi: 10.1111/j.1742-4658.2011.08276.x. PMID:21810173 doi:10.1111/j.1742-4658.2011.08276.x
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