1p5t

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[[Image:1p5t.jpg|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1p5t", creates the "Structure Box" on the page.
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|GENE= mdok1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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{{STRUCTURE_1p5t| PDB=1p5t | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p5t OCA], [http://www.ebi.ac.uk/pdbsum/1p5t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p5t RCSB]</span>
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'''Crystal Structure of Dok1 PTB Domain'''
'''Crystal Structure of Dok1 PTB Domain'''
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[[Category: Yuan, J.]]
[[Category: Yuan, J.]]
[[Category: Zhou, W.]]
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[[Category: signaling protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:43:20 2008''
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Revision as of 01:43, 3 May 2008

Image:1p5t.jpg

Template:STRUCTURE 1p5t

Crystal Structure of Dok1 PTB Domain


Overview

Dok1 is a common substrate of activated protein-tyrosine kinases. It is rapidly tyrosine-phosphorylated in response to receptor tyrosine activation and interacts with ras GTPase-activating protein and Nck, leading to inhibition of ras signaling pathway activation and the c-Jun N-terminal kinase (JNK) and c-Jun activation, respectively. In chronic myelogenous leukemia cells, it has shown constitutive phosphorylation. The N-terminal phosphotyrosine binding (PTB) domain of Dok1 can recognize and bind specifically to phosphotyrosine-containing motifs of receptors. Here we report the crystal structure of the Dok1 PTB domain alone and in complex with a phosphopeptide derived from RET receptor tyrosine kinase. The structure consists of a beta-sandwich composed of two nearly orthogonal, 7-stranded, antiparallel beta-sheets, and it is capped at one side by a C-terminal alpha-helix. The RET phosphopeptide binds to Dok1 via a surface groove formed between strand beta5 and the C-terminal alpha-helix of the PTB domain. The structures reveal the molecular basis for the specific recognition of RET by the Dok1 PTB domain. We also show that Dok1 does not recognize peptide sequences from TrkA and IL-4, which are recognized by Shc and IRS1, respectively.

About this Structure

1P5T is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural basis for the specific recognition of RET by the Dok1 phosphotyrosine binding domain., Shi N, Ye S, Bartlam M, Yang M, Wu J, Liu Y, Sun F, Han X, Peng X, Qiang B, Yuan J, Rao Z, J Biol Chem. 2004 Feb 6;279(6):4962-9. Epub 2003 Nov 7. PMID:14607833 Page seeded by OCA on Sat May 3 04:43:20 2008

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