4ams

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Current revision (11:30, 20 December 2023) (edit) (undo)
 
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== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4ams]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Megavirus_chiliensis Megavirus chiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AMS FirstGlance]. <br>
<table><tr><td colspan='2'>[[4ams]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Megavirus_chiliensis Megavirus chiliensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4AMS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4AMS FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
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<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ams FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ams OCA], [https://pdbe.org/4ams PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ams RCSB], [https://www.ebi.ac.uk/pdbsum/4ams PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ams ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4ams FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4ams OCA], [https://pdbe.org/4ams PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4ams RCSB], [https://www.ebi.ac.uk/pdbsum/4ams PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4ams ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Function ==
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== Publication Abstract from PubMed ==
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[https://www.uniprot.org/uniprot/G5CQN7_9VIRU G5CQN7_9VIRU]
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Mimivirus is the prototype of a new family (the Mimiviridae) of nucleocytoplasmic large DNA viruses (NCLDVs), which already include the Poxviridae, Iridoviridae, Phycodnaviridae and Asfarviridae. Mimivirus specifically replicates in cells from the genus Acanthamoeba. Proteomic analysis of purified mimivirus particles revealed the presence of many subunits of the DNA-directed RNA polymerase II complex. A fully functional pre-transcriptional complex appears to be loaded in the virions, allowing mimivirus to initiate transcription within the host cytoplasm immediately upon infection independently of the host nuclear apparatus. To fully understand this process, a systematic study of mimivirus proteins that are predicted (by bioinformatics) or suspected (by proteomic analysis) to be involved in transcription was initiated by cloning and expressing them in Escherichia coli in order to determine their three-dimensional structures. Here, preliminary crystallographic analysis of the recombinant L544 protein is reported. The crystals belonged to the orthorhombic space group C222(1) with one monomer per asymmetric unit. A MAD data set was used for preliminary phasing using the selenium signal present in a selenomethionine-substituted protein crystal.
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Preliminary crystallographic analysis of a possible transcription factor encoded by the mimivirus L544 gene.,Ciaccafava A, Lartigue A, Mansuelle P, Jeudy S, Abergel C Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Aug 1;67(Pt 8):922-5. doi:, 10.1107/S1744309111020847. Epub 2011 Jul 20. PMID:21821896<ref>PMID:21821896</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 4ams" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
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__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Large Structures]]
[[Category: Large Structures]]
[[Category: Megavirus chiliensis]]
[[Category: Megavirus chiliensis]]
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[[Category: Abergel, C]]
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[[Category: Abergel C]]
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[[Category: Claverie, J M]]
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[[Category: Claverie JM]]
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[[Category: Lartigue, A]]
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[[Category: Lartigue A]]
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[[Category: Priet, S]]
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[[Category: Priet S]]
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[[Category: Mimivirus]]
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[[Category: Transcription coupled dna repair]]
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[[Category: Transferase]]
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Current revision

A Megaviridae ORFan gene encode a new nucleotidyl transferase

PDB ID 4ams

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Proteopedia Page Contributors and Editors (what is this?)

OCA

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