4arn

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of the N-terminal domain of Drosophila Toll receptor

Structural highlights

4arn is a 4 chain structure with sequence from Drosophila melanogaster and Eptatretus burgeri. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.41Å
Ligands:	, , , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TOLL_DROME Q4G1L2_EPTBU

Publication Abstract from PubMed

Drosophila melanogaster Toll is the founding member of an important family of pathogen-recognition receptors in humans, the Toll-like receptor (TLR) family. In contrast, the prototypical receptor is a cytokine-like receptor for Spatzle (Spz) protein and plays a dual role in both development and immunity. Here, we present the crystal structure of the N-terminal domain of the receptor that encompasses the first 201 amino acids at 2.4 A resolution. To our knowledge, the cysteine-rich cap adopts a novel fold unique to Toll-1 orthologs in insects and that is not critical for ligand binding. However, we observed that an antibody directed against the first ten LRRs blocks Spz signaling in a Drosophila cell-based assay. Supplemented by point mutagenesis and deletion analysis, our data suggests that the region up to LRR 14 is involved in Spz binding. Comparison with mammalian TLRs reconciles previous contradictory findings about the mechanism of Toll activation.

Functional insights from the crystal structure of the N-terminal domain of the prototypical toll receptor.,Gangloff M, Arnot CJ, Lewis M, Gay NJ Structure. 2013 Jan 8;21(1):143-53. doi: 10.1016/j.str.2012.11.003. Epub 2012 Dec, 13. PMID:23245851^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Gangloff M, Arnot CJ, Lewis M, Gay NJ. Functional insights from the crystal structure of the N-terminal domain of the prototypical toll receptor. Structure. 2013 Jan 8;21(1):143-53. doi: 10.1016/j.str.2012.11.003. Epub 2012 Dec, 13. PMID:23245851 doi:http://dx.doi.org/10.1016/j.str.2012.11.003

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/4arn"

Categories: Drosophila melanogaster | Eptatretus burgeri | Large Structures | Gangloff M | Gay NJ

@@ Line 3: / Line 3: @@
 <StructureSection load='4arn' size='340' side='right'caption='[[4arn]], [[Resolution|resolution]] 2.41&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[4arn]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARN FirstGlance]. <br>
+<table><tr><td colspan='2'>[[4arn]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster] and [https://en.wikipedia.org/wiki/Eptatretus_burgeri Eptatretus burgeri]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4ARN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4ARN FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.41&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=FUC:ALPHA-L-FUCOSE'>FUC</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4arn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4arn OCA], [https://pdbe.org/4arn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4arn RCSB], [https://www.ebi.ac.uk/pdbsum/4arn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4arn ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/TOLL_DROME TOLL_DROME] [https://www.uniprot.org/uniprot/Q4G1L2_EPTBU Q4G1L2_EPTBU]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 26: @@
 __TOC__
 </StructureSection>
+[[Category: Drosophila melanogaster]]
+[[Category: Eptatretus burgeri]]
 [[Category: Large Structures]]
-[[Category: Gangloff, M]]
+[[Category: Gangloff M]]
-[[Category: Gay, N J]]
+[[Category: Gay NJ]]
-[[Category: Cytokine receptor]]
-[[Category: Embryonic development]]
-[[Category: Immune system]]
-[[Category: Innate immunity]]
-[[Category: Leucine-rich repeat]]
-[[Category: Lrr hybrid technology]]

4arn

From Proteopedia

Current revision

Crystal structure of the N-terminal domain of Drosophila Toll receptor

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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