1p74

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[[Image:1p74.jpg|left|200px]]
[[Image:1p74.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1p74 |SIZE=350|CAPTION= <scene name='initialview01'>1p74</scene>, resolution 2.40&Aring;
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The line below this paragraph, containing "STRUCTURE_1p74", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Shikimate_dehydrogenase Shikimate dehydrogenase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.25 1.1.1.25] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= AROE OR HI0655 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=727 Haemophilus influenzae])
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|DOMAIN=
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{{STRUCTURE_1p74| PDB=1p74 | SCENE= }}
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|RELATEDENTRY=[[1p77|1P77]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p74 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p74 OCA], [http://www.ebi.ac.uk/pdbsum/1p74 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p74 RCSB]</span>
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}}
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'''CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE'''
'''CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE'''
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[[Category: Swanson, R V.]]
[[Category: Swanson, R V.]]
[[Category: Ye, S.]]
[[Category: Ye, S.]]
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[[Category: haemophilus influenzae]]
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[[Category: Haemophilus influenzae]]
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[[Category: shikimate dehydrogenase]]
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[[Category: Shikimate dehydrogenase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:46:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:48 2008''
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Revision as of 01:46, 3 May 2008

Image:1p74.jpg

Template:STRUCTURE 1p74

CRYSTAL STRUCTURE OF SHIKIMATE DEHYDROGENASE (AROE) FROM HAEMOPHILUS INFLUENZAE


Overview

Shikimate dehydrogenase catalyzes the NADPH-dependent reversible reduction of 3-dehydroshikimate to shikimate. We report the first X-ray structure of shikimate dehydrogenase from Haemophilus influenzae to 2.4-A resolution and its complex with NADPH to 1.95-A resolution. The molecule contains two domains, a catalytic domain with a novel open twisted alpha/beta motif and an NADPH binding domain with a typical Rossmann fold. The enzyme contains a unique glycine-rich P-loop with a conserved sequence motif, GAGGXX, that results in NADPH adopting a nonstandard binding mode with the nicotinamide and ribose moieties disordered in the binary complex. A deep pocket with a narrow entrance between the two domains, containing strictly conserved residues primarily contributed by the catalytic domain, is identified as a potential 3-dehydroshikimate binding pocket. The flexibility of the nicotinamide mononucleotide portion of NADPH may be necessary for the substrate 3-dehydroshikimate to enter the pocket and for the release of the product shikimate.

About this Structure

1P74 is a Single protein structure of sequence from Haemophilus influenzae. Full crystallographic information is available from OCA.

Reference

The crystal structure of shikimate dehydrogenase (AroE) reveals a unique NADPH binding mode., Ye S, Von Delft F, Brooun A, Knuth MW, Swanson RV, McRee DE, J Bacteriol. 2003 Jul;185(14):4144-51. PMID:12837789 Page seeded by OCA on Sat May 3 04:46:09 2008

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