1p7d

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[[Image:1p7d.gif|left|200px]]
[[Image:1p7d.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1p7d |SIZE=350|CAPTION= <scene name='initialview01'>1p7d</scene>, resolution 2.95&Aring;
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The line below this paragraph, containing "STRUCTURE_1p7d", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= INT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Enterobacteria phage lambda])
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-->
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|DOMAIN=
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{{STRUCTURE_1p7d| PDB=1p7d | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1p7d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1p7d OCA], [http://www.ebi.ac.uk/pdbsum/1p7d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1p7d RCSB]</span>
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}}
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'''Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA'''
'''Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA'''
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[[Category: Landy, A.]]
[[Category: Landy, A.]]
[[Category: Nunes-Duby, S E.]]
[[Category: Nunes-Duby, S E.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:46:34 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:55:52 2008''
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Revision as of 01:46, 3 May 2008

Image:1p7d.gif

Template:STRUCTURE 1p7d

Crystal structure of the Lambda Integrase (residues 75-356) bound to DNA


Overview

The bacteriophage lambda integrase protein (lambda Int) belongs to a family of tyrosine recombinases that catalyze DNA rearrangements. We have determined a crystal structure of lambda Int complexed with a cleaved DNA substrate through a covalent phosphotyrosine bond. In comparison to an earlier unliganded structure, we observe a drastic conformational change in DNA-bound lambda Int that brings Tyr342 into the active site for cleavage of the DNA in cis. A flexible linker connects the central and the catalytic domains, allowing the protein to encircle the DNA. Binding specificity is achieved through direct interactions with the DNA and indirect readout of the flexibility of the att site. The conformational switch that activates lambda Int for DNA cleavage exposes the C-terminal 8 residues for interactions with a neighboring Int molecule. The protein interactions mediated by lambda Int's C-terminal tail offer a mechanism for the allosteric control of cleavage activity in higher order lambda Int complexes.

About this Structure

1P7D is a Single protein structure of sequence from Enterobacteria phage lambda. Full crystallographic information is available from OCA.

Reference

A conformational switch controls the DNA cleavage activity of lambda integrase., Aihara H, Kwon HJ, Nunes-Duby SE, Landy A, Ellenberger T, Mol Cell. 2003 Jul;12(1):187-98. PMID:12887904 Page seeded by OCA on Sat May 3 04:46:34 2008

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