8hud

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Current revision (23:11, 27 December 2023) (edit) (undo)
 
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'''Unreleased structure'''
 
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The entry 8hud is ON HOLD until Paper Publication
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==Cryo-EM structure of the EvCas9-sgRNA-target DNA ternary complex==
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<StructureSection load='8hud' size='340' side='right'caption='[[8hud]], [[Resolution|resolution]] 3.43&Aring;' scene=''>
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Authors: Tang, N., Wu, Z., Gao, Y., Chen, W., Su, M., Wang, Z., Ji, Q.
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== Structural highlights ==
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<table><tr><td colspan='2'>[[8hud]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Eubacterium_ventriosum_ATCC_27560 Eubacterium ventriosum ATCC 27560] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8HUD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8HUD FirstGlance]. <br>
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Description: Cryo-EM structure of the EvCas9-sgRNA-target DNA ternary complex
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</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.43&#8491;</td></tr>
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[[Category: Unreleased Structures]]
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8hud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8hud OCA], [https://pdbe.org/8hud PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8hud RCSB], [https://www.ebi.ac.uk/pdbsum/8hud PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8hud ProSAT]</span></td></tr>
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[[Category: Gao, Y]]
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</table>
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[[Category: Su, M]]
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== Function ==
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[[Category: Wu, Z]]
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[https://www.uniprot.org/uniprot/A5Z395_9FIRM A5Z395_9FIRM] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer; Cas9 is inactive in the absence of the 2 guide RNAs (gRNA). Cas9 recognizes the protospacer adjacent motif (PAM) in the CRISPR repeat sequences to help distinguish self versus nonself, as targets within the bacterial CRISPR locus do not have PAMs. PAM recognition is also required for catalytic activity.[HAMAP-Rule:MF_01480]
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[[Category: Ji, Q]]
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__TOC__
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[[Category: Chen, W]]
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</StructureSection>
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[[Category: Tang, N]]
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[[Category: Eubacterium ventriosum ATCC 27560]]
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[[Category: Wang, Z]]
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[[Category: Large Structures]]
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[[Category: Synthetic construct]]
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[[Category: Chen W]]
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[[Category: Gao Y]]
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[[Category: Ji Q]]
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[[Category: Su M]]
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[[Category: Tang N]]
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[[Category: Wang Z]]
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[[Category: Wu Z]]

Current revision

Cryo-EM structure of the EvCas9-sgRNA-target DNA ternary complex

PDB ID 8hud

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OCA

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