1paj
From Proteopedia
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'''NMR SOLUTION STRUCTURE AND FLEXIBILITY OF A PEPTIDE ANTIGEN REPRESENTING THE RECEPTOR BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA''' | '''NMR SOLUTION STRUCTURE AND FLEXIBILITY OF A PEPTIDE ANTIGEN REPRESENTING THE RECEPTOR BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA''' | ||
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[[Category: Sonnichsen, F D.]] | [[Category: Sonnichsen, F D.]] | ||
[[Category: Sykes, B D.]] | [[Category: Sykes, B D.]] | ||
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Revision as of 01:53, 3 May 2008
NMR SOLUTION STRUCTURE AND FLEXIBILITY OF A PEPTIDE ANTIGEN REPRESENTING THE RECEPTOR BINDING DOMAIN OF PSEUDOMONAS AERUGINOSA
Overview
A synthetic peptide antigen corresponding to the C-terminus of Pseudomonas aeruginosa K strain pilin has been studied by one and two-dimensional NMR techniques. This peptide exists in two isomeric forms which arise as a result of the I138-P139 amide bond. An ensemble of solution conformations for the trans form of this 17-residue disulfide-bridged peptide (PAK 128-144) has been generated using a simulated annealing procedure in conjunction with distance and torsion angle restraints derived from NMR data. One major class of backbone conformations has been identified for this potential synthetic vaccine and indicates the presence of two beta-turns in the region 134-142. The region that has been established as the epitope for the monoclonal antibody PK99H is consistent with the region of the major conformers that exhibit the most definition in the ensemble (134-140) and also includes a type I beta-turn from residues 134 to 137. The generated structures are also consistent with observed NOEs characteristic of beta-turns and amide proton temperature coefficient data, which indicate the presence of two turns between residues 134 and 142. The presence of secondary structure within the epitope substantiates the theory that immunogenic regions of proteins are those which contain surface-exposed structural elements such as beta-turns. Further implications of the structure on antigenicity and cross-reactivity are discussed.
About this Structure
1PAJ is a Single protein structure of sequence from Pseudomonas aeruginosa. Full crystallographic information is available from OCA.
Reference
NMR solution structure and flexibility of a peptide antigen representing the receptor binding domain of Pseudomonas aeruginosa., McInnes C, Sonnichsen FD, Kay CM, Hodges RS, Sykes BD, Biochemistry. 1993 Dec 14;32(49):13432-40. PMID:8257679 Page seeded by OCA on Sat May 3 04:52:59 2008
