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1yrg

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THE CRYSTAL STRUCTURE OF RNA1P: A NEW FOLD FOR A GTPASE-ACTIVATING PROTEIN

Structural highlights

1yrg is a 2 chain structure with sequence from Schizosaccharomyces pombe. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.66Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

RNA1_SCHPO GTPase activator for the nuclear Ras-related regulatory protein spi1 (Ran), converting it to the putatively inactive GDP-bound state.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

rna1p is the Schizosaccharomyces pombe ortholog of the mammalian GTPase-activating protein (GAP) of Ran. Both proteins are essential for nuclear transport. Here, we report the crystal structure of rna1p at 2.66 A resolution. It contains 11 leucine-rich repeats that adopt the nonglobular shape of a crescent, bearing no resemblance to RhoGAP or RasGAP. The invariant residues of RanGAP form a contiguous surface, strongly indicating the Ran-binding interface. Alanine mutations identify Arg-74 as a critical residue for GTP hydrolysis. In contrast to RasGAP and RhoGAP, Arg-74 could be substituted by lysine and contributed significantly to the binding of Ran. Therefore, we suggest a GAP mechanism for rna1p, which constitutes a variation of the arginine finger mechanism found for Ras GAP and RhoGAP.

The crystal structure of rna1p: a new fold for a GTPase-activating protein.,Hillig RC, Renault L, Vetter IR, Drell T 4th, Wittinghofer A, Becker J Mol Cell. 1999 Jun;3(6):781-91. PMID:10394366^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hillig RC, Renault L, Vetter IR, Drell T 4th, Wittinghofer A, Becker J. The crystal structure of rna1p: a new fold for a GTPase-activating protein. Mol Cell. 1999 Jun;3(6):781-91. PMID:10394366

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1yrg"

@@ Line 3: / Line 3: @@
 <StructureSection load='1yrg' size='340' side='right'caption='[[1yrg]], [[Resolution|resolution]] 2.66&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1yrg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cbs_356 Cbs 356]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YRG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1yrg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schizosaccharomyces_pombe Schizosaccharomyces pombe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YRG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1YRG FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrg OCA], [https://pdbe.org/1yrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yrg RCSB], [https://www.ebi.ac.uk/pdbsum/1yrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrg ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.66&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1yrg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yrg OCA], [https://pdbe.org/1yrg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1yrg RCSB], [https://www.ebi.ac.uk/pdbsum/1yrg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1yrg ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/RNA1_SCHPO RNA1_SCHPO]] GTPase activator for the nuclear Ras-related regulatory protein spi1 (Ran), converting it to the putatively inactive GDP-bound state.
+[https://www.uniprot.org/uniprot/RNA1_SCHPO RNA1_SCHPO] GTPase activator for the nuclear Ras-related regulatory protein spi1 (Ran), converting it to the putatively inactive GDP-bound state.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 31: / Line 32: @@
 __TOC__
 </StructureSection>
-[[Category: Cbs 356]]
 [[Category: Large Structures]]
-[[Category: Becker, J]]
+[[Category: Schizosaccharomyces pombe]]
-[[Category: Drell, T]]
+[[Category: Becker J]]
-[[Category: Hillig, R C]]
+[[Category: Drell T]]
-[[Category: Renault, L]]
+[[Category: Hillig RC]]
-[[Category: Vetter, I R]]
+[[Category: Renault L]]
-[[Category: Wittinghofer, A]]
+[[Category: Vetter IR]]
-[[Category: Gap]]
+[[Category: Wittinghofer A]]
-[[Category: Gtpase-activating protein]]
-[[Category: Hemihedral twinning]]
-[[Category: Leucine-rich repeat protein]]
-[[Category: Lrr]]
-[[Category: Merohedral twinning]]
-[[Category: Merohedry]]
-[[Category: Rangap]]
-[[Category: Rna1p]]
-[[Category: Transcription]]
-[[Category: Twinning]]

1yrg

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Current revision

THE CRYSTAL STRUCTURE OF RNA1P: A NEW FOLD FOR A GTPASE-ACTIVATING PROTEIN

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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