1pd7
From Proteopedia
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[[Image:1pd7.jpg|left|200px]] | [[Image:1pd7.jpg|left|200px]] | ||
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'''Extended SID of Mad1 bound to the PAH2 domain of mSin3B''' | '''Extended SID of Mad1 bound to the PAH2 domain of mSin3B''' | ||
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[[Category: Stunnenberg, H G.]] | [[Category: Stunnenberg, H G.]] | ||
[[Category: Vuister, G W.]] | [[Category: Vuister, G W.]] | ||
| - | [[Category: | + | [[Category: Eukaryotic transcriptional regulation]] |
| - | [[Category: | + | [[Category: Mad1]] |
| - | [[Category: | + | [[Category: Pah2]] |
| - | [[Category: | + | [[Category: Protein-protein interaction]] |
| - | [[Category: | + | [[Category: Sin3]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 04:57:21 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 01:57, 3 May 2008
Extended SID of Mad1 bound to the PAH2 domain of mSin3B
Overview
Sin3 forms the scaffold for a multiprotein corepressor complex that silences transcription via the action of histone deacetylases. Sin3 is recruited to the DNA by several DNA binding repressors, such as the helix-loop-helix proteins of the Mad family. Here, we elaborate on the Mad-Sin3 interaction based on a binding study, solution structure, and dynamics of the PAH2 domain of mSin3 in complex to an extended Sin3 interacting domain (SID) of 24 residues of Mad1. We show that SID residues Met7 and Glu23, outside the previously defined minimal binding motif, mediate additional hydrophobic and electrostatic interactions with PAH2. On the basis of these results we propose an extended consensus sequence describing the PAH2-SID interaction specifically for the Mad family, showing that residues outside the hydrophobic core of the SID interact with PAH2 and modulate binding affinity to appropriate levels.
About this Structure
1PD7 is a Protein complex structure of sequences from Mus musculus. Full crystallographic information is available from OCA.
Reference
Extension of the binding motif of the Sin3 interacting domain of the Mad family proteins., van Ingen H, Lasonder E, Jansen JF, Kaan AM, Spronk CA, Stunnenberg HG, Vuister GW, Biochemistry. 2004 Jan 13;43(1):46-54. PMID:14705930 Page seeded by OCA on Sat May 3 04:57:21 2008
