3ews

From Proteopedia

(Difference between revisions)

Current revision

Human DEAD-box RNA-helicase DDX19 in complex with ADP

Structural highlights

3ews is a 2 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.7Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DD19B_HUMAN ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

DEXD/H-box RNA helicases couple ATP hydrolysis to RNA remodeling by an unknown mechanism. We used x-ray crystallography and biochemical analysis of the human DEXD/H-box protein DDX19 to investigate its regulatory mechanism. The crystal structures of DDX19, in its RNA-bound prehydrolysis and free posthydrolysis state, reveal an alpha-helix that inserts between the conserved domains of the free protein to negatively regulate ATPase activity. This finding was corroborated by biochemical data that confirm an autoregulatory function of the N-terminal region of the protein. This is the first study describing crystal structures of a DEXD/H-box protein in its open and closed cleft conformations.

The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch.,Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Collins R, Karlberg T, Lehtio L, Schutz P, van den Berg S, Dahlgren LG, Hammarstrom M, Weigelt J, Schuler H. The DEXD/H-box RNA helicase DDX19 is regulated by an {alpha}-helical switch. J Biol Chem. 2009 Apr 17;284(16):10296-300. Epub 2009 Feb 25. PMID:19244245 doi:10.1074/jbc.C900018200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/3ews"

@@ Line 3: / Line 3: @@
 <StructureSection load='3ews' size='340' side='right'caption='[[3ews]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[3ews]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EWS FirstGlance]. <br>
+<table><tr><td colspan='2'>[[3ews]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3EWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3EWS FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">DBP5, DDX19, DDX19B ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene></td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3ews FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ews OCA], [https://pdbe.org/3ews PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3ews RCSB], [https://www.ebi.ac.uk/pdbsum/3ews PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3ews ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[https://www.uniprot.org/uniprot/DD19B_HUMAN DD19B_HUMAN]] ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
+[https://www.uniprot.org/uniprot/DD19B_HUMAN DD19B_HUMAN] ATP-dependent RNA helicase involved in mRNA export from the nucleus. Rather than unwinding RNA duplexes, DDX19B functions as a remodeler of ribonucleoprotein particles, whereby proteins bound to nuclear mRNA are dissociated and replaced by cytoplasmic mRNA binding proteins.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
@@ Line 36: / Line 36: @@
 __TOC__
 </StructureSection>
-[[Category: Human]]
+[[Category: Homo sapiens]]
 [[Category: Large Structures]]
-[[Category: Andersson, j]]
+[[Category: Andersson j]]
-[[Category: Arrowsmith, c h]]
+[[Category: Arrowsmith ch]]
-[[Category: Berglund, h]]
+[[Category: Berglund h]]
-[[Category: Bountra, c]]
+[[Category: Bountra c]]
-[[Category: Collins, r]]
+[[Category: Collins r]]
-[[Category: Dahlgren, l g]]
+[[Category: Dahlgren lg]]
-[[Category: Edwards, a m]]
+[[Category: Edwards am]]
-[[Category: Flodin, s]]
+[[Category: Flodin s]]
-[[Category: Flores, a]]
+[[Category: Flores a]]
-[[Category: Graslund, s]]
+[[Category: Graslund s]]
-[[Category: Hammarstrom, m]]
+[[Category: Hammarstrom m]]
-[[Category: Johansson, a]]
+[[Category: Johansson a]]
-[[Category: Johansson, i]]
+[[Category: Johansson i]]
-[[Category: Karlberg, t]]
+[[Category: Karlberg t]]
-[[Category: Kotenyova, t]]
+[[Category: Kotenyova t]]
-[[Category: Lehtio, l]]
+[[Category: Lehtio l]]
-[[Category: Moche, m]]
+[[Category: Moche m]]
-[[Category: Nilsson, m e]]
+[[Category: Nilsson me]]
-[[Category: Nordlund, p]]
+[[Category: Nordlund p]]
-[[Category: Nyman, t]]
+[[Category: Nyman t]]
-[[Category: Olesen, k]]
+[[Category: Olesen k]]
-[[Category: Persson, c]]
+[[Category: Persson c]]
-[[Category: Structural genomic]]
+[[Category: Sagemark j]]
-[[Category: Sagemark, j]]
+[[Category: Schueler h]]
-[[Category: Schueler, h]]
+[[Category: Thorsell ag]]
-[[Category: Thorsell, a g]]
+[[Category: Tresaugues l]]
-[[Category: Tresaugues, l]]
+[[Category: Van den berg s]]
-[[Category: Weigelt, j]]
+[[Category: Weigelt j]]
-[[Category: Welin, m]]
+[[Category: Welin m]]
-[[Category: Wikstrom, m]]
+[[Category: Wikstrom m]]
-[[Category: Wisniewska, m]]
+[[Category: Wisniewska m]]
-[[Category: Berg, s Van den]]
-[[Category: Adp]]
-[[Category: Alternative splicing]]
-[[Category: Atp-binding]]
-[[Category: Cytoplasm]]
-[[Category: Dead]]
-[[Category: Helicase]]
-[[Category: Hydrolase]]
-[[Category: Membrane]]
-[[Category: Mrna]]
-[[Category: Mrna transport]]
-[[Category: Nuclear pore complex]]
-[[Category: Nucleotide-binding]]
-[[Category: Nucleus]]
-[[Category: Protein transport]]
-[[Category: Rna helicase]]
-[[Category: Rna-binding]]
-[[Category: Rrna]]
-[[Category: Sgc]]
-[[Category: Translocation]]
-[[Category: Transport]]

3ews

From Proteopedia

Current revision

Human DEAD-box RNA-helicase DDX19 in complex with ADP

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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