5ow3

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of a C-terminally truncated trimeric ectodomain of the Arabidopsis thaliana gamete fusion protein HAP2

Structural highlights

5ow3 is a 1 chain structure with sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.75Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

HAP2_ARATH Required for male fertility (PubMed:17079265, PubMed:20333238). Plays a role in pollen tube guidance and successful gamete attachment (PubMed:17079265). Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm (PubMed:17079265, PubMed:20333238, PubMed:21209845). Mediates the fusion of cell membranes (PubMed:28137780). Not required for pollen tube outgrowth (PubMed:17079265, PubMed:20333238).^[1] ^[2] ^[3] ^[4] ^[5] ^[6]

Publication Abstract from PubMed

HAPLESS2 (HAP2) is a broadly conserved, gamete-expressed transmembrane protein that was shown recently to be structurally homologous to viral class II fusion proteins, which initiate fusion with host cells via insertion of fusion loops into the host membrane. However, the functional conformation of the HAP2 fusion loops has remained unknown, as the reported X-ray structure of Chlamydomonas reinhardtii HAP2 lacked this critical region. Here, we report a structure-guided alignment that reveals diversification of the proposed HAP2 fusion loops. Representative crystal structures show that in flowering plants, HAP2 has a single prominent fusion loop projecting an amphipathic helix at its apex, while in trypanosomes, three small nonpolar loops of HAP2 are poised to interact with the target membrane. A detailed structure-function analysis of the Arabidopsis HAP2 amphipathic fusion helix defines key residues that are essential for membrane insertion and for gamete fusion. Our study suggests that HAP2 may have evolved multiple modes of membrane insertion to accommodate the diversity of membrane environments it has encountered during eukaryotic evolution.

Evolutionary diversification of the HAP2 membrane insertion motifs to drive gamete fusion across eukaryotes.,Fedry J, Forcina J, Legrand P, Pehau-Arnaudet G, Haouz A, Johnson M, Rey FA, Krey T PLoS Biol. 2018 Aug 13;16(8):e2006357. doi: 10.1371/journal.pbio.2006357., eCollection 2018 Aug. PMID:30102690^[7]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Mori T, Kuroiwa H, Higashiyama T, Kuroiwa T. GENERATIVE CELL SPECIFIC 1 is essential for angiosperm fertilization. Nat Cell Biol. 2006 Jan;8(1):64-71. doi: 10.1038/ncb1345. Epub 2005 Dec 25. PMID:16378100 doi:http://dx.doi.org/10.1038/ncb1345
↑ von Besser K, Frank AC, Johnson MA, Preuss D. Arabidopsis HAP2 (GCS1) is a sperm-specific gene required for pollen tube guidance and fertilization. Development. 2006 Dec;133(23):4761-9. doi: 10.1242/dev.02683. Epub 2006 Nov 1. PMID:17079265 doi:http://dx.doi.org/10.1242/dev.02683
↑ Wong JL, Leydon AR, Johnson MA. HAP2(GCS1)-dependent gamete fusion requires a positively charged carboxy-terminal domain. PLoS Genet. 2010 Mar 19;6(3):e1000882. doi: 10.1371/journal.pgen.1000882. PMID:20333238 doi:http://dx.doi.org/10.1371/journal.pgen.1000882
↑ Mori T, Hirai M, Kuroiwa T, Miyagishima SY. The functional domain of GCS1-based gamete fusion resides in the amino terminus in plant and parasite species. PLoS One. 2010 Dec 31;5(12):e15957. doi: 10.1371/journal.pone.0015957. PMID:21209845 doi:http://dx.doi.org/10.1371/journal.pone.0015957
↑ Sprunck S, Rademacher S, Vogler F, Gheyselinck J, Grossniklaus U, Dresselhaus T. Egg cell-secreted EC1 triggers sperm cell activation during double fertilization. Science. 2012 Nov 23;338(6110):1093-7. doi: 10.1126/science.1223944. PMID:23180860 doi:http://dx.doi.org/10.1126/science.1223944
↑ Valansi C, Moi D, Leikina E, Matveev E, Grana M, Chernomordik LV, Romero H, Aguilar PS, Podbilewicz B. Arabidopsis HAP2/GCS1 is a gamete fusion protein homologous to somatic and viral fusogens. J Cell Biol. 2017 Mar 6;216(3):571-581. doi: 10.1083/jcb.201610093. Epub 2017 Jan, 30. PMID:28137780 doi:http://dx.doi.org/10.1083/jcb.201610093
↑ Fedry J, Forcina J, Legrand P, Pehau-Arnaudet G, Haouz A, Johnson M, Rey FA, Krey T. Evolutionary diversification of the HAP2 membrane insertion motifs to drive gamete fusion across eukaryotes. PLoS Biol. 2018 Aug 13;16(8):e2006357. doi: 10.1371/journal.pbio.2006357., eCollection 2018 Aug. PMID:30102690 doi:http://dx.doi.org/10.1371/journal.pbio.2006357

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5ow3"

@@ Line 3: / Line 3: @@
 <StructureSection load='5ow3' size='340' side='right'caption='[[5ow3]], [[Resolution|resolution]] 2.75&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5ow3]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OW3 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5OW3 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5ow3]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Arabidopsis_thaliana Arabidopsis thaliana]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OW3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OW3 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">HAP2, GCS1, At4g11720, T5C23 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5ow3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ow3 OCA], [http://pdbe.org/5ow3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ow3 RCSB], [http://www.ebi.ac.uk/pdbsum/5ow3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ow3 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ow3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ow3 OCA], [https://pdbe.org/5ow3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ow3 RCSB], [https://www.ebi.ac.uk/pdbsum/5ow3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ow3 ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/HAP2_ARATH HAP2_ARATH]] Required for male fertility (PubMed:17079265, PubMed:20333238). Plays a role in pollen tube guidance and successful gamete attachment (PubMed:17079265). Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm (PubMed:17079265, PubMed:20333238, PubMed:21209845). Mediates the fusion of cell membranes (PubMed:28137780). Not required for pollen tube outgrowth (PubMed:17079265, PubMed:20333238).<ref>PMID:16378100</ref> <ref>PMID:17079265</ref> <ref>PMID:20333238</ref> <ref>PMID:21209845</ref> <ref>PMID:23180860</ref> <ref>PMID:28137780</ref>
+[https://www.uniprot.org/uniprot/HAP2_ARATH HAP2_ARATH] Required for male fertility (PubMed:17079265, PubMed:20333238). Plays a role in pollen tube guidance and successful gamete attachment (PubMed:17079265). Essential for the fusion of gametes during double fertilization, where one male gamete fuses with the egg to produce a zygote, and another male gamete fuses with the central cell to produce the endosperm (PubMed:17079265, PubMed:20333238, PubMed:21209845). Mediates the fusion of cell membranes (PubMed:28137780). Not required for pollen tube outgrowth (PubMed:17079265, PubMed:20333238).<ref>PMID:16378100</ref> <ref>PMID:17079265</ref> <ref>PMID:20333238</ref> <ref>PMID:21209845</ref> <ref>PMID:23180860</ref> <ref>PMID:28137780</ref>
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Arath]]
+[[Category: Arabidopsis thaliana]]
 [[Category: Large Structures]]
-[[Category: Fedry, J]]
+[[Category: Fedry J]]
-[[Category: Krey, T]]
+[[Category: Krey T]]
-[[Category: Legrand, P]]
+[[Category: Legrand P]]
-[[Category: Rey, F A]]
+[[Category: Rey FA]]
-[[Category: Class ii fold]]
-[[Category: Gamete fusion protein]]
-[[Category: Hap2]]
-[[Category: Membrane fusion]]
-[[Category: Membrane protein]]

5ow3

From Proteopedia

Current revision

Crystal structure of a C-terminally truncated trimeric ectodomain of the Arabidopsis thaliana gamete fusion protein HAP2

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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