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| | ==Crystal structure of NeqN/NeqC complex from Nanoarcheaum equitans at 1.7A== | | ==Crystal structure of NeqN/NeqC complex from Nanoarcheaum equitans at 1.7A== |
| - | <StructureSection load='5oxx' size='340' side='right' caption='[[5oxx]], [[Resolution|resolution]] 1.74Å' scene=''> | + | <StructureSection load='5oxx' size='340' side='right'caption='[[5oxx]], [[Resolution|resolution]] 1.74Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5oxx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Microarchaeum_sp._kin4-m Microarchaeum sp. kin4-m]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OXX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5oxx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Nanoarchaeum_equitans_Kin4-M Nanoarchaeum equitans Kin4-M]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OXX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OXX FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">NEQ068 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228908 Microarchaeum sp. Kin4-M]), NEQ528 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=228908 Microarchaeum sp. Kin4-M])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.74Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5oxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxx OCA], [http://pdbe.org/5oxx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5oxx RCSB], [http://www.ebi.ac.uk/pdbsum/5oxx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxx ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5oxx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5oxx OCA], [https://pdbe.org/5oxx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5oxx RCSB], [https://www.ebi.ac.uk/pdbsum/5oxx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5oxx ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q74N74_NANEQ Q74N74_NANEQ] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Microarchaeum sp. kin4-m]] | + | [[Category: Large Structures]] |
| - | [[Category: Aparicio, D]] | + | [[Category: Nanoarchaeum equitans Kin4-M]] |
| - | [[Category: Fita, I]] | + | [[Category: Aparicio D]] |
| - | [[Category: Perez-Luque, R]] | + | [[Category: Fita I]] |
| - | [[Category: Ribo, M]] | + | [[Category: Perez-Luque R]] |
| - | [[Category: Intein complex]]
| + | [[Category: Ribo M]] |
| - | [[Category: Splicing]]
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| Structural highlights
Function
Q74N74_NANEQ
Publication Abstract from PubMed
Split inteins are expressed as two separated subunits (N-intein and C-intein) fused to the corresponding exteins. The specific association of both intein subunits precedes protein splicing, which results in excision of the intein subunits and in ligation, by a peptide bond, of the concomitant exteins. Catalytically active intein precursors are typically too reactive for crystallization or even isolation. Neq pol is the trans-intein of the B-type DNA polymerase I split gene from hyperthermophile Nanoarchaeum equitans. We have determined the crystal structures of both the isolated NeqN and the complex of NeqN and NeqC subunits carrying the wild-type sequences, including the essential catalytic residues Ser1 and Thr+1, in addition to seven and three residues of the N- and C-exteins, respectively. These structures provide detailed information on the unique oxyester chemistry of the splicing mechanism of Neq pol and of the extensive rearrangements that occur in NeqN during the association step.
Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein.,Gordo V, Aparicio D, Perez-Luque R, Benito A, Vilanova M, Uson I, Fita I, Ribo M Cell Chem Biol. 2018 Jul 19;25(7):871-879.e2. doi:, 10.1016/j.chembiol.2018.04.008. Epub 2018 May 10. PMID:29754955[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Gordo V, Aparicio D, Perez-Luque R, Benito A, Vilanova M, Uson I, Fita I, Ribo M. Structural Insights into Subunits Assembly and the Oxyester Splicing Mechanism of Neq pol Split Intein. Cell Chem Biol. 2018 Jul 19;25(7):871-879.e2. doi:, 10.1016/j.chembiol.2018.04.008. Epub 2018 May 10. PMID:29754955 doi:http://dx.doi.org/10.1016/j.chembiol.2018.04.008
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