8pzk
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Gloeocapsa sp. PCC 7428== | |
| + | <StructureSection load='8pzk' size='340' side='right'caption='[[8pzk]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8pzk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Gloeocapsa_sp._PCC_7428 Gloeocapsa sp. PCC 7428]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8PZK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8PZK FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AZI:AZIDE+ION'>AZI</scene>, <scene name='pdbligand=CSO:S-HYDROXYCYSTEINE'>CSO</scene>, <scene name='pdbligand=ECH:BETA,BETA-CAROTEN-4-ONE'>ECH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8pzk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8pzk OCA], [https://pdbe.org/8pzk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8pzk RCSB], [https://www.ebi.ac.uk/pdbsum/8pzk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8pzk ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/K9XH36_9CHRO K9XH36_9CHRO] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The Orange Carotenoid Protein (OCP) is a unique photoreceptor crucial for cyanobacterial photoprotection. Best studied Synechocystis sp. PCC 6803 OCP belongs to the large OCP1 family. Downregulated by the Fluorescence Recovery Protein (FRP) in low-light, high-light-activated OCP1 binds to the phycobilisomes and performs non-photochemical quenching. Recently discovered families OCP2 and OCP3 remain structurally and functionally underexplored, and no systematic comparative studies have ever been conducted. Here we present two first crystal structures of OCP2 from morphoecophysiologically different cyanobacteria and provide their comprehensive structural, spectroscopic and functional comparison with OCP1, the recently described OCP3 and all-OCP ancestor. Structures enable correlation of spectroscopic signatures with the effective number of hydrogen and discovered here chalcogen bonds anchoring the ketocarotenoid in OCP, as well as with the rotation of the echinenone's beta-ionone ring in the CTD. Structural data also helped rationalize the observed differences in OCP/FRP and OCP/phycobilisome functional interactions. These data are expected to foster OCP research and applications in optogenetics, targeted carotenoid delivery and cyanobacterial biomass engineering. | ||
| - | + | Structural framework for the understanding spectroscopic and functional signatures of the cyanobacterial Orange Carotenoid Protein families.,Sluchanko NN, Maksimov EG, Slonimskiy YB, Varfolomeeva LA, Bukhanko AY, Egorkin NA, Tsoraev GV, Khrenova MG, Ge B, Qin S, Boyko KM, Popov VO Int J Biol Macromol. 2024 Jan;254(Pt 2):127874. doi: , 10.1016/j.ijbiomac.2023.127874. Epub 2023 Nov 7. PMID:37939760<ref>PMID:37939760</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| - | [[Category: | + | <div class="pdbe-citations 8pzk" style="background-color:#fffaf0;"></div> |
| - | [[Category: | + | == References == |
| - | [[Category: | + | <references/> |
| - | [[Category: | + | __TOC__ |
| - | [[Category: | + | </StructureSection> |
| - | [[Category: | + | [[Category: Gloeocapsa sp. PCC 7428]] |
| + | [[Category: Large Structures]] | ||
| + | [[Category: Boyko KM]] | ||
| + | [[Category: Maksimov EG]] | ||
| + | [[Category: Popov VO]] | ||
| + | [[Category: Slonimskiy YB]] | ||
| + | [[Category: Sluchanko NN]] | ||
| + | [[Category: Varfolomeeva LA]] | ||
Revision as of 11:03, 3 January 2024
Crystal structure of the Orange Carotenoid Protein 2 (OCP2) from Gloeocapsa sp. PCC 7428
| |||||||||||
