5hm4

From Proteopedia

(Difference between revisions)

Current revision

Crystal structure of oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.0 A resolution

Structural highlights

5hm4 is a 1 chain structure with sequence from Thermotoga maritima. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q9X0V3_THEMA

Publication Abstract from PubMed

The ligand-induced conformational changes of periplasmic binding proteins (PBP) play a key role in the acquisition of metabolites in ATP binding cassette (ABC) transport systems. This conformational change allows for differential recognition of the ligand occupancy of the PBP by the ABC transporter. This minimizes futile ATP hydrolysis in the transporter, a phenomenon in which ATP hydrolysis is not coupled to metabolite transport. In many systems, the PBP conformational change is insufficient at eliminating futile ATP hydrolysis. Here we identify an additional state of the PBP that is also allosterically regulated by the ligand. Ligand binding to the homodimeric apo PBP leads to a tightening of the interface alpha-helices so that the hydrogen bonding pattern shifts to that of a 310 helix, in-turn altering the contacts and the dynamics of the protein interface so that the monomer exists in the presence of ligand.

Periplasmic Binding Protein Dimer Has a Second Allosteric Event Tied to Ligand Binding.,Li L, Ghimire-Rijal S, Lucas SL, Stanley CB, Wright E, Agarwal PK, Myles DA, Cuneo MJ Biochemistry. 2017 Oct 10;56(40):5328-5337. doi: 10.1021/acs.biochem.7b00657., Epub 2017 Sep 22. PMID:28876049^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Li L, Ghimire-Rijal S, Lucas SL, Stanley CB, Wright E, Agarwal PK, Myles DA, Cuneo MJ. Periplasmic Binding Protein Dimer Has a Second Allosteric Event Tied to Ligand Binding. Biochemistry. 2017 Oct 10;56(40):5328-5337. doi: 10.1021/acs.biochem.7b00657., Epub 2017 Sep 22. PMID:28876049 doi:http://dx.doi.org/10.1021/acs.biochem.7b00657

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5hm4"

@@ Line 3: / Line 3: @@
 <StructureSection load='5hm4' size='340' side='right'caption='[[5hm4]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5hm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_43589 Atcc 43589]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HM4 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5hm4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima Thermotoga maritima]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5HM4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5HM4 FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4pfu|4pfu]], [[4pfw|4pfw]]</div></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Tmari_1233 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2336 ATCC 43589])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5hm4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5hm4 OCA], [https://pdbe.org/5hm4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5hm4 RCSB], [https://www.ebi.ac.uk/pdbsum/5hm4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5hm4 ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q9X0V3_THEMA Q9X0V3_THEMA]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Atcc 43589]]
 [[Category: Large Structures]]
-[[Category: Cuneo, M J]]
+[[Category: Thermotoga maritima]]
-[[Category: Ghimire-Rijal, S]]
+[[Category: Cuneo MJ]]
-[[Category: Lu, X]]
+[[Category: Ghimire-Rijal S]]
-[[Category: Myles, D A.A]]
+[[Category: Lu X]]
-[[Category: Abc transporter]]
+[[Category: Myles DAA]]
-[[Category: Transport protein]]

5hm4

From Proteopedia

Current revision

Crystal structure of oligopeptide ABC transporter, periplasmic oligopeptide-binding protein (TM1226) from THERMOTOGA MARITIMA at 2.0 A resolution

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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