8w7p
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Extracellular domain of a sensor histidine kinase== | |
| + | <StructureSection load='8w7p' size='340' side='right'caption='[[8w7p]], [[Resolution|resolution]] 1.80Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8w7p]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Paenibacillus_sp._FPU-7 Paenibacillus sp. FPU-7]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8W7P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8W7P FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8w7p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8w7p OCA], [https://pdbe.org/8w7p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8w7p RCSB], [https://www.ebi.ac.uk/pdbsum/8w7p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8w7p ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | We have previously isolated the Gram-positive chitin-degrading bacterium Paenibacillus sp. str. FPU-7. This bacterium traps chitin disaccharide (GlcNAc)2 on its cell surface using two homologous solute-binding proteins, NagB1 and NagB2. Bacteria use histidine kinase of the two-component regulatory system as an extracellular environment sensor. In this study, we found that nagS, which encodes a histidine kinase, is located next to the nagB1 gene. Biochemical experiments revealed that the NagS sensor domain (NagS30-294) interacts with the NagB1-(GlcNAc)2 complex. However, proof of NagS30-294 interacting with NagB1 without (GlcNAc)2 is currently unavailable. In contrast to NagB1, no complex formation was observed between NagS30-294 and NagB2, even in the presence of (GlcNAc)2. The NagS30-294 crystal structure at 1.8 A resolution suggested that the canonical tandem-Per-Arnt-Sim (PAS) fold recognizes the NagB1-(GlcNAc)2 complex. This study provides insight into the recognition of chitin oligosaccharides by bacteria. | ||
| - | + | Characterization of the extracellular domain of sensor histidine kinase NagS from Paenibacillus sp. str. FPU-7: nagS interacts with oligosaccharide binding protein NagB1 in complexes with N, N'-diacetylchitobiose.,Itoh T, Ogawa T, Hibi T, Kimoto H Biosci Biotechnol Biochem. 2023 Dec 6:zbad173. doi: 10.1093/bbb/zbad173. PMID:38059852<ref>PMID:38059852</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8w7p" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Paenibacillus sp. FPU-7]] | ||
| + | [[Category: Hibi T]] | ||
| + | [[Category: Itoh T]] | ||
| + | [[Category: Kimoto H]] | ||
| + | [[Category: Ogawa T]] | ||
Revision as of 10:28, 10 January 2024
Extracellular domain of a sensor histidine kinase
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