1pib
From Proteopedia
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[[Image:1pib.gif|left|200px]] | [[Image:1pib.gif|left|200px]] | ||
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'''Solution structure of DNA containing CPD opposited by GA''' | '''Solution structure of DNA containing CPD opposited by GA''' | ||
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==About this Structure== | ==About this Structure== | ||
| - | + | Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1PIB OCA]. | |
==Reference== | ==Reference== | ||
NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15121904 15121904] | NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/15121904 15121904] | ||
| - | [[Category: Protein complex]] | ||
[[Category: Choi, B S.]] | [[Category: Choi, B S.]] | ||
[[Category: Lee, J H.]] | [[Category: Lee, J H.]] | ||
[[Category: Park, C J.]] | [[Category: Park, C J.]] | ||
[[Category: Shin, J S.]] | [[Category: Shin, J S.]] | ||
| - | [[Category: | + | [[Category: Cis-syn cyclobutane pyrimidine dimer]] |
| - | [[Category: | + | [[Category: Cpd containing dna]] |
| - | [[Category: | + | [[Category: Helical distortion]] |
| - | [[Category: | + | [[Category: Thyminedimer]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:06:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:06, 3 May 2008
Solution structure of DNA containing CPD opposited by GA
Overview
The cis-syn cyclobutane pyrimidine dimer (CPD) is a cytotoxic, mutagenic and carcinogenic DNA photoproduct and is repaired by the nucleotide excision repair (NER) pathway in mammalian cells. The XPC-hHR23B complex as the initiator of global genomic NER binds to sites of certain kinds of DNA damage. Although CPDs are rarely recognized by the XPC-hHR23B complex, the presence of mismatched bases opposite a CPD significantly increased the binding affinity of the XPC-hHR23B complex to the CPD. In order to decipher the properties of the DNA structures that determine the binding affinity for XPC-hHR23B to DNA, we carried out structural analyses of the various types of CPDs by NMR spectroscopy. The DNA duplex which contains a single 3' T*G wobble pair in a CPD (CPD/GA duplex) induces little conformational distortion. However, severe distortion of the helical conformation occurs when a CPD contains double T*G wobble pairs (CPD/GG duplex) even though the T residues of the CPD form stable hydrogen bonds with the opposite G residues. The helical bending angle of the CPD/GG duplex was larger than those of the CPD/GA duplex and properly matched CPD/AA duplex. The fluctuation of the backbone conformation and significant changes in the widths of the major and minor grooves at the double T*G wobble paired site were also observed in the CPD/GG duplex. These structural features were also found in a duplex that contains the (6-4) adduct, which is efficiently recognized by the XPC-hHR23B complex. Thus, we suggest that the unique structural features of the DNA double helix (that is, helical bending, flexible backbone conformation, and significant changes of the major and/or minor grooves) might be important factors in determining the binding affinity of the XPC-hHR23B complex to DNA.
About this Structure
Full crystallographic information is available from OCA.
Reference
NMR structure of the DNA decamer duplex containing double T*G mismatches of cis-syn cyclobutane pyrimidine dimer: implications for DNA damage recognition by the XPC-hHR23B complex., Lee JH, Park CJ, Shin JS, Ikegami T, Akutsu H, Choi BS, Nucleic Acids Res. 2004 Apr 30;32(8):2474-81. Print 2004. PMID:15121904 Page seeded by OCA on Sat May 3 05:06:45 2008
