8gjc
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==X-ray crystallographic structure of a beta-hairpin peptide derived from Abeta 17-35. (ORN)LVFFAED(ORN)GAI(N-Me-Ile)GLM== | |
| + | <StructureSection load='8gjc' size='340' side='right'caption='[[8gjc]], [[Resolution|resolution]] 1.43Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[8gjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=8GJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=8GJC FirstGlance]. <br> | ||
| + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.431Å</td></tr> | ||
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IML:N-METHYL-ISOLEUCINE'>IML</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=ORN:L-ORNITHINE'>ORN</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=TFA:TRIFLUOROACETIC+ACID'>TFA</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=8gjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=8gjc OCA], [https://pdbe.org/8gjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=8gjc RCSB], [https://www.ebi.ac.uk/pdbsum/8gjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=8gjc ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Amyloid-beta (Abeta) forms heterogeneous oligomers, which are implicated in the pathogenesis of Alzheimer's disease (AD). Many Abeta oligomers consist of beta-hairpin building blocks horizontal line Abeta peptides in beta-hairpin conformations. beta-Hairpins of Abeta can adopt a variety of alignments, but the role that beta-hairpin alignment plays in the formation and heterogeneity of Abeta oligomers is poorly understood. To explore the effect of beta-hairpin alignment on the oligomerization of Abeta peptides, we designed and studied two model peptides with two different beta-hairpin alignments. Peptides Abetam(17-36) and Abetam(17-35) mimic two different beta-hairpins that Abeta can form, the Abeta(17-36) and Abeta(17-35) beta-hairpins, respectively. These hairpins are similar in composition but differ in hairpin alignment, altering the facial arrangements of the side chains of the residues that they contain. X-ray crystallography and SDS-PAGE demonstrate that the difference in facial arrangement between these peptides leads to distinct oligomer formation. In the crystal state, Abetam(17-36) forms triangular trimers that further assemble to form hexamers, while Abetam(17-35) forms tetrameric beta-barrels. In SDS-PAGE, Abetam(17-36) assembles to form a ladder of oligomers, while Abetam(17-35) either assembles to form a dimer or does not assemble at all. The differences in the behavior of Abetam(17-36) and Abetam(17-35) suggest beta-hairpin alignment as a source of the observed heterogeneity of Abeta oligomers. | ||
| - | + | beta-Hairpin Alignment Alters Oligomer Formation in Abeta-Derived Peptides.,Ruttenberg SM, Kreutzer AG, Truex NL, Nowick JS Biochemistry. 2024 Jan 16;63(2):212-218. doi: 10.1021/acs.biochem.3c00526. Epub , 2024 Jan 1. PMID:38163326<ref>PMID:38163326</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 8gjc" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
| + | [[Category: Synthetic construct]] | ||
| + | [[Category: Kreutzer AG]] | ||
| + | [[Category: Nowick JS]] | ||
| + | [[Category: Ruttenberg SM]] | ||
Current revision
X-ray crystallographic structure of a beta-hairpin peptide derived from Abeta 17-35. (ORN)LVFFAED(ORN)GAI(N-Me-Ile)GLM
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