1pkv

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[[Image:1pkv.jpg|left|200px]]
[[Image:1pkv.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 1pkv |SIZE=350|CAPTION= <scene name='initialview01'>1pkv</scene>, resolution 2.60&Aring;
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The line below this paragraph, containing "STRUCTURE_1pkv", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=RBF:RIBOFLAVINE'>RBF</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Riboflavin_synthase Riboflavin synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.9 2.5.1.9] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= RIBE OR RIBC OR B1662 OR SF1690 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1pkv| PDB=1pkv | SCENE= }}
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|RELATEDENTRY=[[1hze|1HZE]], [[1i8d|1I8D]], [[1kzl|1KZL]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pkv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pkv OCA], [http://www.ebi.ac.uk/pdbsum/1pkv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pkv RCSB]</span>
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}}
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'''The N-terminal domain of riboflavin synthase in complex with riboflavin'''
'''The N-terminal domain of riboflavin synthase in complex with riboflavin'''
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[[Category: Ladenstein, R.]]
[[Category: Ladenstein, R.]]
[[Category: Meining, W.]]
[[Category: Meining, W.]]
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[[Category: beta-barrel]]
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[[Category: Beta-barrel]]
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[[Category: dimer]]
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[[Category: Dimer]]
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[[Category: greek key motif]]
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[[Category: Greek key motif]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:12:03 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:01:05 2008''
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Revision as of 02:12, 3 May 2008

Image:1pkv.jpg

Template:STRUCTURE 1pkv

The N-terminal domain of riboflavin synthase in complex with riboflavin


Overview

Riboflavin synthase of Escherichia coli is a homotrimer with a molecular mass of 70 kDa. The enzyme catalyzes the dismutation of 6,7-dimethyl-8-(1'-D-ribityl)-lumazine, affording riboflavin and 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione. The N-terminal segment (residues 1-87) and the C-terminal segment (residues 98-187) form beta-barrels with similar fold and a high degree of sequence similarity. A recombinant peptide comprising amino acid residues 1-97 forms a dimer, which binds riboflavin with high affinity. Here, we report the structure of this construct in complex with riboflavin at 2.6A resolution. It is demonstrated that the complex can serve as a model for ligand-binding in the native enzyme. The structure and riboflavin-binding mode is in excellent agreement with structural information obtained from the native enzyme from Escherichia coli and riboflavin synthase from Schizosaccharomyces pombe. The implications for the binding specificity and the regiospecificity of the catalyzed reaction are discussed.

About this Structure

1PKV is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

The structure of the N-terminal domain of riboflavin synthase in complex with riboflavin at 2.6A resolution., Meining W, Eberhardt S, Bacher A, Ladenstein R, J Mol Biol. 2003 Aug 29;331(5):1053-63. PMID:12927541 Page seeded by OCA on Sat May 3 05:12:03 2008

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