1pmo

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[[Image:1pmo.gif|left|200px]]
[[Image:1pmo.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1pmo |SIZE=350|CAPTION= <scene name='initialview01'>1pmo</scene>, resolution 2.3&Aring;
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The line below this paragraph, containing "STRUCTURE_1pmo", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=PLR:(5-HYDROXY-4,6-DIMETHYLPYRIDIN-3-YL)METHYL+DIHYDROGEN+PHOSPHATE'>PLR</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glutamate_decarboxylase Glutamate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.15 4.1.1.15] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= GADB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1pmo| PDB=1pmo | SCENE= }}
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|RELATEDENTRY=[[1pmm|1PMM]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pmo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pmo OCA], [http://www.ebi.ac.uk/pdbsum/1pmo PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pmo RCSB]</span>
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}}
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'''Crystal structure of Escherichia coli GadB (neutral pH)'''
'''Crystal structure of Escherichia coli GadB (neutral pH)'''
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[[Category: Grutter, M G.]]
[[Category: Grutter, M G.]]
[[Category: Gut, H.]]
[[Category: Gut, H.]]
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[[Category: neutral-ph form of gadb]]
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[[Category: Neutral-ph form of gadb]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:15:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:01:41 2008''
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Revision as of 02:15, 3 May 2008

Image:1pmo.gif

Template:STRUCTURE 1pmo

Crystal structure of Escherichia coli GadB (neutral pH)


Overview

Glutamate decarboxylase is a vitamin B6-dependent enzyme, which catalyses the decarboxylation of glutamate to gamma-aminobutyrate. In Escherichia coli, expression of glutamate decarboxylase (GadB), a 330 kDa hexamer, is induced to maintain the physiological pH under acidic conditions, like those of the passage through the stomach en route to the intestine. GadB, together with the antiporter GadC, constitutes the gad acid resistance system, which confers the ability for bacterial survival for at least 2 h in a strongly acidic environment. GadB undergoes a pH-dependent conformational change and exhibits an activity optimum at low pH. We determined the crystal structures of GadB at acidic and neutral pH. They reveal the molecular details of the conformational change and the structural basis for the acidic pH optimum. We demonstrate that the enzyme is localized exclusively in the cytoplasm at neutral pH, but is recruited to the membrane when the pH falls. We show by structure-based site-directed mutagenesis that the triple helix bundle formed by the N-termini of the protein at acidic pH is the major determinant for this behaviour.

About this Structure

1PMO is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Crystal structure and functional analysis of Escherichia coli glutamate decarboxylase., Capitani G, De Biase D, Aurizi C, Gut H, Bossa F, Grutter MG, EMBO J. 2003 Aug 15;22(16):4027-37. PMID:12912902 Page seeded by OCA on Sat May 3 05:15:19 2008

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