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| | <StructureSection load='6hnk' size='340' side='right'caption='[[6hnk]], [[Resolution|resolution]] 2.50Å' scene=''> | | <StructureSection load='6hnk' size='340' side='right'caption='[[6hnk]], [[Resolution|resolution]] 2.50Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6hnk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Clod6 Clod6]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HNK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6hnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Clostridioides_difficile_630 Clostridioides difficile 630]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HNK FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CD630_08730 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=272563 CLOD6])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hnk OCA], [http://pdbe.org/6hnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hnk RCSB], [http://www.ebi.ac.uk/pdbsum/6hnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hnk ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6hnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hnk OCA], [https://pdbe.org/6hnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6hnk RCSB], [https://www.ebi.ac.uk/pdbsum/6hnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6hnk ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q18A65_CLOD6 Q18A65_CLOD6] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Clod6]] | + | [[Category: Clostridioides difficile 630]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Acharya, K R]] | + | [[Category: Acharya KR]] |
| - | [[Category: Bradshaw, W J]] | + | [[Category: Bradshaw WJ]] |
| - | [[Category: Harmer, N J]] | + | [[Category: Harmer NJ]] |
| - | [[Category: Kovacs-Simon, A]] | + | [[Category: Kovacs-Simon A]] |
| - | [[Category: Michell, S L]] | + | [[Category: Michell SL]] |
| - | [[Category: Adhesin]]
| + | |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Substrate binding protein]]
| + | |
| - | [[Category: Transport protein]]
| + | |
| - | [[Category: Tyrosine binding protein]]
| + | |
| Structural highlights
Function
Q18A65_CLOD6
Publication Abstract from PubMed
Clostridioides difficile is the primary cause of antibiotic-associated diarrhoea and colitis, a healthcare-associated intestinal disease resulting in a significant fatality rate. Colonization of the gut is critical for C. difficile pathogenesis, and the bacterial molecules essential for efficient colonization therefore offer great potential as vaccine candidates. Here we present findings demonstrating that the C. difficile immunogenic lipoprotein CD0873 plays a critical role in pathogen success in vivo. We found that in a dixenic colonization model, a CD0873-positive strain of C. difficile significantly outcompeted a CD0873-negative strain. Immunization of mice with recombinant CD0873 prevented long-term gut colonization and was correlated with a strong secretory IgA immune response. We further present high-resolution crystal structures of CD0873, at 1.80-2.50 A resolutions, offering a first view of the ligand-binding pocket of CD0873 and provide evidence that this lipoprotein adhesin is part of a tyrosine import system, an amino acid key in C. difficile infection. These findings suggest that CD0873 could serve as a effective component in a vaccine against C. difficile.
Molecular features of lipoprotein CD0873 - a potential vaccine against the human pathogen Clostridioides difficile.,Bradshaw WJ, Bruxelle JF, Kovacs-Simon A, Harmer NJ, Janoir C, Pechine S, Acharya KR, Michell SL J Biol Chem. 2019 Aug 16. pii: RA119.010120. doi: 10.1074/jbc.RA119.010120. PMID:31420448[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bradshaw WJ, Bruxelle JF, Kovacs-Simon A, Harmer NJ, Janoir C, Pechine S, Acharya KR, Michell SL. Molecular features of lipoprotein CD0873 - a potential vaccine against the human pathogen Clostridioides difficile. J Biol Chem. 2019 Aug 16. pii: RA119.010120. doi: 10.1074/jbc.RA119.010120. PMID:31420448 doi:http://dx.doi.org/10.1074/jbc.RA119.010120
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