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6htl

From Proteopedia

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Measles Phosphoprotein Coiled-Coil Domain IPKI Variant

Structural highlights

6htl is a 1 chain structure with sequence from Measles morbillivirus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 2.3Å
Ligands:
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

Q83623_9MONO

Publication Abstract from PubMed

The polymerase of negative-stranded RNA viruses consists of the large protein (L) and the phosphoprotein (P), the latter serving both as a chaperon and a cofactor for L. We mapped within measles virus (MeV) P the regions responsible for binding and stabilizing L and showed that the coiled-coil multimerization domain (MD) of P is required for gene expression. MeV MD is kinked as a result of the presence of a stammer. Both restoration of the heptad regularity and displacement of the stammer strongly decrease or abrogate activity in a minigenome assay. By contrast, P activity is rather tolerant of substitutions within the stammer. Single substitutions at the "a" or "d" hydrophobic anchor positions with residues of variable hydrophobicity revealed that P functionality requires a narrow range of cohesiveness of its MD. Results collectively indicate that, beyond merely ensuring P oligomerization, the MD finely tunes viral gene expression through its cohesiveness.

Regulation of measles virus gene expression by P protein coiled-coil properties.,Bloyet LM, Schramm A, Lazert C, Raynal B, Hologne M, Walker O, Longhi S, Gerlier D Sci Adv. 2019 May 8;5(5):eaaw3702. doi: 10.1126/sciadv.aaw3702. eCollection 2019 , May. PMID:31086822^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bloyet LM, Schramm A, Lazert C, Raynal B, Hologne M, Walker O, Longhi S, Gerlier D. Regulation of measles virus gene expression by P protein coiled-coil properties. Sci Adv. 2019 May 8;5(5):eaaw3702. doi: 10.1126/sciadv.aaw3702. eCollection 2019 , May. PMID:31086822 doi:http://dx.doi.org/10.1126/sciadv.aaw3702

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6htl"

Categories: Large Structures | Measles morbillivirus | Longhi S | Schramm A

@@ Line 3: / Line 3: @@
 <StructureSection load='6htl' size='340' side='right'caption='[[6htl]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6htl]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HTL FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6htl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Measles_morbillivirus Measles morbillivirus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HTL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6HTL FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6htl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htl OCA], [http://pdbe.org/6htl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6htl RCSB], [http://www.ebi.ac.uk/pdbsum/6htl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6htl ProSAT]</span></td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6htl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6htl OCA], [https://pdbe.org/6htl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6htl RCSB], [https://www.ebi.ac.uk/pdbsum/6htl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6htl ProSAT]</span></td></tr>
 </table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q83623_9MONO Q83623_9MONO]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 21: / Line 24: @@
 </StructureSection>
 [[Category: Large Structures]]
-[[Category: Longhi, S]]
+[[Category: Measles morbillivirus]]
-[[Category: Schramm, A]]
+[[Category: Longhi S]]
-[[Category: 3-10 helix]]
+[[Category: Schramm A]]
-[[Category: Alpha helix]]
-[[Category: Coiled-coil]]
-[[Category: Tetramer]]
-[[Category: Viral protein]]

6htl

From Proteopedia

Current revision

Measles Phosphoprotein Coiled-Coil Domain IPKI Variant

Structural highlights

Function

Publication Abstract from PubMed

References

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Proteopedia Page Contributors and Editors (what is this?)

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