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| | <StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20Å' scene=''> | | <StructureSection load='6i2z' size='340' side='right'caption='[[6i2z]], [[Resolution|resolution]] 3.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Borpe Borpe]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6I2Z FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6i2z]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bordetella_pertussis_Tohama_I Bordetella pertussis Tohama I]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I2Z OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I2Z FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.2Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4uiq|4uiq]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEM:PROTOPORPHYRIN+IX+CONTAINING+FE'>HEM</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [http://pdbe.org/6i2z PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [http://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i2z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i2z OCA], [https://pdbe.org/6i2z PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i2z RCSB], [https://www.ebi.ac.uk/pdbsum/6i2z PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i2z ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q7VTL8_BORPE Q7VTL8_BORPE] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Borpe]] | + | [[Category: Bordetella pertussis Tohama I]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Berghmans, H]] | + | [[Category: Berghmans H]] |
| - | [[Category: Bolognesi, M]] | + | [[Category: Bolognesi M]] |
| - | [[Category: Bruno, S]] | + | [[Category: Bruno S]] |
| - | [[Category: Cuypers, B]] | + | [[Category: Cuypers B]] |
| - | [[Category: Dewilde, S]] | + | [[Category: De Schutter A]] |
| - | [[Category: Doorslaer, S Van]] | + | [[Category: Dewilde S]] |
| - | [[Category: Germani, F]] | + | [[Category: Germani F]] |
| - | [[Category: Hauwaert, M L.Van]]
| + | [[Category: Moens L]] |
| - | [[Category: Moens, L]] | + | [[Category: Mozzarelli A]] |
| - | [[Category: Mozzarelli, A]] | + | [[Category: Pesce A]] |
| - | [[Category: Pesce, A]] | + | [[Category: Van Doorslaer S]] |
| - | [[Category: Schutter, A De]] | + | [[Category: Van Hauwaert M-L]] |
| - | [[Category: Biofilm formation]] | + | |
| - | [[Category: C-di-gmp and enzyme specificity]]
| + | |
| - | [[Category: Heme-based sensor]]
| + | |
| - | [[Category: Oxygen affinity]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
Q7VTL8_BORPE
Publication Abstract from PubMed
AIMS: Structural and functional characterization of the globin coupled sensors (GCS) of <i>Azotobacter vinelandii </i>(AvGReg) and <i>Bordetella pertussis</i> (BpeGReg). RESULTS: UV/Vis and RR spectroscopies confirm the presence in <i>Av</i>GReg and <i>Bpe</i>GReg of a globin domain capable of reversible gaseous ligand binding. In <i>Av</i>GReg an influence of the transmitter domain on the heme proximal region of the globin domain can be seen, and <i>k'</i>CO is higher than for other GCSs. The O<sub>2</sub> binding kinetics suggest the presence of an open and a closed conformation. As for <i>Bpe</i>GReg, the fully oxygenated <i>Av</i>GReg show a very high DGC activity. The CO rebinding to <i>Bpe</i>GReg indicates that intra- and intermolecular interactions influence the ligand binding. The globin domains of both proteins (<i>Av</i>GReg-Gb and <i>Bpe</i>Greg-Gb*) share the same GCS fold, a similar proximal but a different distal side structure. They homodimerize through a G-H helical bundle as in other GCS. However, <i>Bpe</i>GReg-Gb* shows also a second dimerization mode. INNOVATION: This paper extends our knowledge on the GCS proteins and contributes to a better understanding of the GCSs' role in the formation of bacterial biofilms. CONCLUSIONS: <i>Av</i>GReg and <i>Bpe</i>Greg conform to the GCS family, share a similar overall structure but they have different properties in terms of the ligand binding. In particular, <i>Av</i>Greg shows an open and closed conformation that in the latter form will very tightly bind oxygen. <i>Bpe</i>GReg has only one closed conformation. In both proteins, it is the fully oxygenated GCS form that catalyzes the production of the second messenger.
Structural and functional characterization of the globin-coupled sensors of <i>Azotobacter vinelandii </i>and <i>Bordetella pertussis</i>.,Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Germani F, Nardini M, De Schutter A, Cuypers B, Berghmans H, Van Hauwaert ML, Stefano B, Mozzarelli A, Moens L, Van Doorslaer S, Bolognesi M, Pesce A, Dewilde S. Structural and functional characterization of the globin-coupled sensors of Azotobacter vinelandii and Bordetella pertussis. Antioxid Redox Signal. 2019 Sep 27. doi: 10.1089/ars.2018.7690. PMID:31559835 doi:http://dx.doi.org/10.1089/ars.2018.7690
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