1pn8

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[[Image:1pn8.gif|left|200px]]
[[Image:1pn8.gif|left|200px]]
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{{Structure
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|PDB= 1pn8 |SIZE=350|CAPTION= <scene name='initialview01'>1pn8</scene>
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The line below this paragraph, containing "STRUCTURE_1pn8", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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{{STRUCTURE_1pn8| PDB=1pn8 | SCENE= }}
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|RELATEDENTRY=[[1gix|1GIX]], [[1giy|1GIY]], [[1pn6|1PN6]], [[1pn7|1PN7]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pn8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pn8 OCA], [http://www.ebi.ac.uk/pdbsum/1pn8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pn8 RCSB]</span>
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'''Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.'''
'''Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.'''
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[[Category: Valle, M.]]
[[Category: Valle, M.]]
[[Category: Zavialov, A.]]
[[Category: Zavialov, A.]]
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[[Category: ribosomal protein]]
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[[Category: Ribosomal protein]]
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[[Category: trna]]
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[[Category: Trna]]
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[[Category: trna binding protein]]
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[[Category: Trna binding protein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:16:23 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:01:52 2008''
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Revision as of 02:16, 3 May 2008

Image:1pn8.gif

Template:STRUCTURE 1pn8

Coordinates of S12, L11 proteins and E-site tRNA from 70S crystal structure separately fitted into the Cryo-EM map of E.coli 70S.EF-G.GDPNP complex. The atomic coordinates originally from the E-site tRNA were fitted in the position of the hybrid P/E-site tRNA.


Overview

During the ribosomal translocation, the binding of elongation factor G (EF-G) to the pretranslocational ribosome leads to a ratchet-like rotation of the 30S subunit relative to the 50S subunit in the direction of the mRNA movement. By means of cryo-electron microscopy we observe that this rotation is accompanied by a 20 A movement of the L1 stalk of the 50S subunit, implying that this region is involved in the translocation of deacylated tRNAs from the P to the E site. These ribosomal motions can occur only when the P-site tRNA is deacylated. Prior to peptidyl-transfer to the A-site tRNA or peptide removal, the presence of the charged P-site tRNA locks the ribosome and prohibits both of these motions.

About this Structure

1PN8 is a Protein complex structure of sequences from Thermotoga maritima and Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Locking and unlocking of ribosomal motions., Valle M, Zavialov A, Sengupta J, Rawat U, Ehrenberg M, Frank J, Cell. 2003 Jul 11;114(1):123-34. PMID:12859903 Page seeded by OCA on Sat May 3 05:16:23 2008

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