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| | <StructureSection load='6i50' size='340' side='right'caption='[[6i50]], [[Resolution|resolution]] 1.69Å' scene=''> | | <StructureSection load='6i50' size='340' side='right'caption='[[6i50]], [[Resolution|resolution]] 1.69Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6i50]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I50 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6I50 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6i50]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Spodoptera_frugiperda Spodoptera frugiperda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6I50 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6I50 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6i50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i50 OCA], [http://pdbe.org/6i50 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6i50 RCSB], [http://www.ebi.ac.uk/pdbsum/6i50 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6i50 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.69Å</td></tr> |
| | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6i50 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6i50 OCA], [https://pdbe.org/6i50 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6i50 RCSB], [https://www.ebi.ac.uk/pdbsum/6i50 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6i50 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A2H1VTZ0_SPOFR A0A2H1VTZ0_SPOFR] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| | [[Category: Spodoptera frugiperda]] | | [[Category: Spodoptera frugiperda]] |
| - | [[Category: Bertinelli, M]] | + | [[Category: Bertinelli M]] |
| - | [[Category: Grimes, J M]] | + | [[Category: Grimes JM]] |
| - | [[Category: Paesen, G C]] | + | [[Category: Paesen GC]] |
| - | [[Category: Renner, M]] | + | [[Category: Renner M]] |
| - | [[Category: Apoptosis]]
| + | |
| - | [[Category: Arthropod]]
| + | |
| - | [[Category: Cytokine]]
| + | |
| - | [[Category: Tnf]]
| + | |
| - | [[Category: Tumour necrosis factor]]
| + | |
| Structural highlights
Function
A0A2H1VTZ0_SPOFR
Publication Abstract from PubMed
The tumour necrosis factor alpha (TNFalpha) superfamily of proteins are critical in numerous biological processes, such as in development and immunity. Eiger is the sole TNFalpha member described in arthropods such as in the important model organism Drosophila. To date there are no structural data on any Eiger protein. Here we present the structure of the TNF domain of Eiger from the fall armyworm Spodoptera frugiperda (SfEiger) to 1.7 A from a serendipitously obtained crystal without prior knowledge of the protein sequence. Our structure confirms that canonical trimerization is conserved from ancestral TNFs and points towards a mode of receptor engagement. Furthermore, we observe numerous surface histidines on SfEiger, potentially acting as pH switches following internalization into endosomes. Our data contributes to the genome annotation of S. frugiperda, a voracious agricultural pest, and can serve as a basis for future structure-function investigations of the TNF system in related arthropods such as Drosophila.
High-resolution crystal structure of arthropod Eiger TNF suggests a mode of receptor engagement and altered surface charge within endosomes.,Bertinelli M, Paesen GC, Grimes JM, Renner M Commun Biol. 2019 Aug 6;2:293. doi: 10.1038/s42003-019-0541-0. eCollection 2019. PMID:31396573[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Bertinelli M, Paesen GC, Grimes JM, Renner M. High-resolution crystal structure of arthropod Eiger TNF suggests a mode of receptor engagement and altered surface charge within endosomes. Commun Biol. 2019 Aug 6;2:293. doi: 10.1038/s42003-019-0541-0. eCollection 2019. PMID:31396573 doi:http://dx.doi.org/10.1038/s42003-019-0541-0
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