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| | <StructureSection load='6qfd' size='340' side='right'caption='[[6qfd]], [[Resolution|resolution]] 2.13Å' scene=''> | | <StructureSection load='6qfd' size='340' side='right'caption='[[6qfd]], [[Resolution|resolution]] 2.13Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6qfd]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Halsa Halsa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6QFD FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6qfd]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Halobacterium_salinarum_NRC-1 Halobacterium salinarum NRC-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6QFD OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6QFD FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.133Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VNG_0258H ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=64091 HALSA])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6qfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfd OCA], [http://pdbe.org/6qfd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6qfd RCSB], [http://www.ebi.ac.uk/pdbsum/6qfd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfd ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6qfd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6qfd OCA], [https://pdbe.org/6qfd PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6qfd RCSB], [https://www.ebi.ac.uk/pdbsum/6qfd PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6qfd ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q9HSF4_HALSA Q9HSF4_HALSA] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Halsa]] | + | [[Category: Halobacterium salinarum NRC-1]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kutnowski, N]] | + | [[Category: Kutnowski N]] |
| - | [[Category: Shaanan, B]] | + | [[Category: Shaanan B]] |
| - | [[Category: Dna binding protein]]
| + | |
| - | [[Category: Halophile]]
| + | |
| - | [[Category: High salt medium]]
| + | |
| - | [[Category: Protein-dna interaction]]
| + | |
| - | [[Category: Rosr]]
| + | |
| - | [[Category: Whth dna binding protein]]
| + | |
| Structural highlights
Function
Q9HSF4_HALSA
Publication Abstract from PubMed
Interactions between proteins and DNA are crucial for all biological systems. Many studies have shown the dependence of protein-DNA interactions on the surrounding salt concentration. How these interactions are maintained in the hypersaline environments that halophiles inhabit remains puzzling. Towards solving this enigma, we identified the DNA motif recognized by the Halobactrium salinarum ROS-dependent transcription factor (hsRosR), determined the structure of several hsRosR-DNA complexes and investigated the DNA-binding process under extreme high-salt conditions. The picture that emerges from this work contributes to our understanding of the principles underlying the interplay between electrostatic interactions and salt-mediated protein-DNA interactions in an ionic environment characterized by molar salt concentrations.
Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.,Kutnowski N, Shmulevich F, Davidov G, Shahar A, Bar-Zvi D, Eichler J, Zarivach R, Shaanan B Nucleic Acids Res. 2019 Jul 16. pii: 5532612. doi: 10.1093/nar/gkz604. PMID:31310308[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Kutnowski N, Shmulevich F, Davidov G, Shahar A, Bar-Zvi D, Eichler J, Zarivach R, Shaanan B. Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR. Nucleic Acids Res. 2019 Jul 16. pii: 5532612. doi: 10.1093/nar/gkz604. PMID:31310308 doi:http://dx.doi.org/10.1093/nar/gkz604
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