6s0c
From Proteopedia
(Difference between revisions)
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==Crystal structure of methionine gamma-lyase from Citrobacter freundii modified by dimethylthiosulfinate== | ==Crystal structure of methionine gamma-lyase from Citrobacter freundii modified by dimethylthiosulfinate== | ||
| - | <StructureSection load='6s0c' size='340' side='right'caption='[[6s0c]]' scene=''> | + | <StructureSection load='6s0c' size='340' side='right'caption='[[6s0c]], [[Resolution|resolution]] 1.46Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0C OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[6s0c]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Citrobacter_freundii Citrobacter freundii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S0C OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S0C FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.46Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene>, <scene name='pdbligand=SCH:S-METHYL-THIO-CYSTEINE'>SCH</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s0c OCA], [https://pdbe.org/6s0c PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s0c RCSB], [https://www.ebi.ac.uk/pdbsum/6s0c PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s0c ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/A0A0A5P8W7_CITFR A0A0A5P8W7_CITFR] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Interactions of Citrobacter freundii methionine gamma-lyase (MGL) with sulfoxides of typical substrates were investigated. It was found that sulfoxides are suicide substrates of the enzyme. The products of the beta- and gamma-elimination reactions of sulfoxides, thiosulfinates, oxidize three cysteine residues of the enzyme. Three-dimensional structures of MGL inactivated by dimethyl thiosulfinate and diethyl thiosulfinate were determined at 1.46A and 1.59A resolution. Analysis of the structures identified SH groups oxidized by thiosulfinates and revealed the structural bases of MGL inactivation. The extent of inactivation of MGL in the catalysis of the beta-elimination reaction depends on the length of the <<tail>> at oxidized Cys115. Oxidation of Cys115 results in MGL incapable to catalyze the stage of methyl mercaptan elimination of the physiological reaction. | ||
| + | |||
| + | Sulfoxides of sulfur-containing amino acids are suicide substrates of Citrobacter freundii methionine gamma-lyase. Structural bases of the enzyme inactivation.,Revtovich S, Morozova E, Kulikova V, Koval V, Anufrieva N, Nikulin A, Demidkina T Biochimie. 2020 Jan;168:190-197. doi: 10.1016/j.biochi.2019.11.004. Epub 2019 Nov, 9. PMID:31711941<ref>PMID:31711941</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6s0c" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Cystathionine gamma synthase|Cystathionine gamma synthase]] | ||
| + | *[[Methionine gamma-lyase 3D structures|Methionine gamma-lyase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Citrobacter freundii]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Demidkina TV]] | [[Category: Demidkina TV]] | ||
Current revision
Crystal structure of methionine gamma-lyase from Citrobacter freundii modified by dimethylthiosulfinate
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