6s2b
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Structure of beta-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosyl-erythritol== | ==Structure of beta-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosyl-erythritol== | ||
| - | <StructureSection load='6s2b' size='340' side='right'caption='[[6s2b]]' scene=''> | + | <StructureSection load='6s2b' size='340' side='right'caption='[[6s2b]], [[Resolution|resolution]] 1.88Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S2B FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6s2b]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Schwanniomyces_occidentalis Schwanniomyces occidentalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6S2B OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6S2B FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s2b OCA], [https://pdbe.org/6s2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s2b RCSB], [https://www.ebi.ac.uk/pdbsum/6s2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s2b ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.88Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=KTE:(2~{S},3~{R})-4-[(2~{R},3~{S},4~{S},5~{R})-2,5-bis(hydroxymethyl)-3,4-bis(oxidanyl)oxolan-2-yl]oxybutane-1,2,3-triol'>KTE</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6s2b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6s2b OCA], [https://pdbe.org/6s2b PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6s2b RCSB], [https://www.ebi.ac.uk/pdbsum/6s2b PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6s2b ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/E5D0X5_SCHOC E5D0X5_SCHOC] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The beta-fructofuranosidase from Schwanniomyces occidentalis (Ffase) is a useful biotechnological tool for the fructosylation of different acceptors to produce fructooligosaccharides (FOS) and fructo-conjugates. In this work, the structural determinants of Ffase involved in the transfructosylating reaction of the alditols mannitol and erythritol have been studied in detail. Complexes with fructosyl-erythritol or sucrose were analyzed by crystallography and the effect of mutational changes in positions Gln-176, Gln-228, and Asn-254 studied to explore their role in modulating this biocatalytic process. Interestingly, N254T variant enhanced the wild-type protein production of fructosyl-erythritol and FOS by [Formula: see text] 30% and 48%, respectively. Moreover, it produced neokestose, which represented [Formula: see text] 27% of total FOS, and yielded 31.8 g l(-1) blastose by using glucose as exclusive fructosyl-acceptor. Noteworthy, N254D and Q176E replacements turned the specificity of Ffase transferase activity towards the synthesis of the fructosylated polyols at the expense of FOS production, but without increasing the total reaction efficiency. The results presented here highlight the relevance of the pair Gln-228/Asn-254 for Ffase donor-sucrose binding and opens new windows of opportunity for optimizing the generation of fructosyl-derivatives by this enzyme enhancing its biotechnological applicability. | ||
| + | |||
| + | New insights into the molecular mechanism behind mannitol and erythritol fructosylation by beta-fructofuranosidase from Schwanniomyces occidentalis.,Rodrigo-Frutos D, Jimenez-Ortega E, Piedrabuena D, Ramirez-Escudero M, Miguez N, Plou FJ, Sanz-Aparicio J, Fernandez-Lobato M Sci Rep. 2021 Mar 30;11(1):7158. doi: 10.1038/s41598-021-86568-6. PMID:33785821<ref>PMID:33785821</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6s2b" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Schwanniomyces occidentalis]] | ||
[[Category: Jimenez-Ortega E]] | [[Category: Jimenez-Ortega E]] | ||
[[Category: Sanz-Aparicio J]] | [[Category: Sanz-Aparicio J]] | ||
Current revision
Structure of beta-fructofuranosidase from Schwanniomyces occidentalis complexed with fructosyl-erythritol
| |||||||||||
