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| | <StructureSection load='6sbg' size='340' side='right'caption='[[6sbg]], [[Resolution|resolution]] 2.30Å' scene=''> | | <StructureSection load='6sbg' size='340' side='right'caption='[[6sbg]], [[Resolution|resolution]] 2.30Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sbg]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Scytonema_sp._pcc_10023 Scytonema sp. pcc 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6SBG FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sbg]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Scytonema_sp._PCC_10023 Scytonema sp. PCC 10023]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SBG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SBG FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=L4E:geranylgeranyl+dihydroxybenzoate'>L4E</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sbb|6sbb]]</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=L4E:geranylgeranyl+dihydroxybenzoate'>L4E</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mstE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1680591 Scytonema sp. PCC 10023])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbg OCA], [https://pdbe.org/6sbg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sbg RCSB], [https://www.ebi.ac.uk/pdbsum/6sbg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbg ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6sbg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sbg OCA], [http://pdbe.org/6sbg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sbg RCSB], [http://www.ebi.ac.uk/pdbsum/6sbg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sbg ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/A0A2D1CM82_9CYAN A0A2D1CM82_9CYAN] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Scytonema sp. pcc 10023]] | + | [[Category: Scytonema sp. PCC 10023]] |
| - | [[Category: Cahn, J K.B]] | + | [[Category: Cahn JKB]] |
| - | [[Category: Ecker, F]] | + | [[Category: Ecker F]] |
| - | [[Category: Groll, M]] | + | [[Category: Groll M]] |
| - | [[Category: Leopold-Messer, S]] | + | [[Category: Leopold-Messer S]] |
| - | [[Category: Moosmann, P]] | + | [[Category: Moosmann P]] |
| - | [[Category: Piel, J]] | + | [[Category: Piel J]] |
| - | [[Category: Alpha6-alpha6 barrel]]
| + | |
| - | [[Category: Biosynthetic protein]]
| + | |
| - | [[Category: Marine drug]]
| + | |
| - | [[Category: Merosterol]]
| + | |
| - | [[Category: Type ii terpene cyclase]]
| + | |
| Structural highlights
Function
A0A2D1CM82_9CYAN
Publication Abstract from PubMed
Class II terpene cyclases, such as oxidosqualene and squalene-hopene cyclases, catalyse some of the most complex polycyclization reactions. They minimally exhibit a beta,gamma-didomain architecture that has been evolutionarily repurposed in a wide range of terpene-processing enzymes and likely resulted from a fusion of unidentified monodomain proteins. Although single domain class I terpene cyclases have already been identified, the corresponding class II counterparts have not been previously reported. Here we present high-resolution X-ray structures of a monodomain class II cyclase, merosterolic acid synthase (MstE). With a minimalistic beta-domain architecture, this cyanobacterial enzyme is able to construct four rings in cytotoxic meroterpenoids with a sterol-like topology. The structures with bound substrate, product, and inhibitor provide detailed snapshots of a cyclization mechanism largely governed by residues located in a noncanonical enzyme region. Our results complement the few known class II cyclase crystal structures, while also indicating that archaic monodomain cyclases might have already catalyzed complex reaction cascades.
A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.,Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Moosmann P, Ecker F, Leopold-Messer S, Cahn JKB, Dieterich CL, Groll M, Piel J. A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds. Nat Chem. 2020 Aug 10. pii: 10.1038/s41557-020-0515-3. doi:, 10.1038/s41557-020-0515-3. PMID:32778689 doi:http://dx.doi.org/10.1038/s41557-020-0515-3
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