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| | <StructureSection load='6sji' size='340' side='right'caption='[[6sji]], [[Resolution|resolution]] 1.80Å' scene=''> | | <StructureSection load='6sji' size='340' side='right'caption='[[6sji]], [[Resolution|resolution]] 1.80Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sji]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Thioalkalivibrio_paradoxus_arh_1 Thioalkalivibrio paradoxus arh 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SJI FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sji]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Thioalkalivibrio_paradoxus_ARh_1 Thioalkalivibrio paradoxus ARh 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SJI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SJI FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">THITH_13335 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=713585 Thioalkalivibrio paradoxus ARh 1])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sji OCA], [http://pdbe.org/6sji PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sji RCSB], [http://www.ebi.ac.uk/pdbsum/6sji PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sji ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sji OCA], [https://pdbe.org/6sji PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sji RCSB], [https://www.ebi.ac.uk/pdbsum/6sji PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sji ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/W0DP94_9GAMM W0DP94_9GAMM] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Thioalkalivibrio paradoxus arh 1]] | + | [[Category: Thioalkalivibrio paradoxus ARh 1]] |
| - | [[Category: Osipov, E]] | + | [[Category: Osipov E]] |
| - | [[Category: Polyakov, K M]] | + | [[Category: Polyakov KM]] |
| - | [[Category: Popov, V O]] | + | [[Category: Popov VO]] |
| - | [[Category: Rakitina, T V]] | + | [[Category: Rakitina TV]] |
| - | [[Category: Tikhonova, T V]] | + | [[Category: Tikhonova TV]] |
| - | [[Category: Copper center]]
| + | |
| - | [[Category: Oxidoreductase]]
| + | |
| - | [[Category: Thiocyanate dehydrogenase]]
| + | |
| Structural highlights
Function
W0DP94_9GAMM
Publication Abstract from PubMed
Biocatalytic copper centers are generally involved in the activation and reduction of dioxygen, with only few exceptions known. Here we report the discovery and characterization of a previously undescribed copper center that forms the active site of a copper-containing enzyme thiocyanate dehydrogenase (suggested EC 1.8.2.7) that was purified from the haloalkaliphilic sulfur-oxidizing bacterium of the genus Thioalkalivibrio ubiquitous in saline alkaline soda lakes. The copper cluster is formed by three copper ions located at the corners of a near-isosceles triangle and facilitates a direct thiocyanate conversion into cyanate, elemental sulfur, and two reducing equivalents without involvement of molecular oxygen. A molecular mechanism of catalysis is suggested based on high-resolution three-dimensional structures, electron paramagnetic resonance (EPR) spectroscopy, quantum mechanics/molecular mechanics (QM/MM) simulations, kinetic studies, and the results of site-directed mutagenesis.
Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase.,Tikhonova TV, Sorokin DY, Hagen WR, Khrenova MG, Muyzer G, Rakitina TV, Shabalin IG, Trofimov AA, Tsallagov SI, Popov VO Proc Natl Acad Sci U S A. 2020 Feb 24. pii: 1922133117. doi:, 10.1073/pnas.1922133117. PMID:32094184[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Tikhonova TV, Sorokin DY, Hagen WR, Khrenova MG, Muyzer G, Rakitina TV, Shabalin IG, Trofimov AA, Tsallagov SI, Popov VO. Trinuclear copper biocatalytic center forms an active site of thiocyanate dehydrogenase. Proc Natl Acad Sci U S A. 2020 Feb 24. pii: 1922133117. doi:, 10.1073/pnas.1922133117. PMID:32094184 doi:http://dx.doi.org/10.1073/pnas.1922133117
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