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| | <StructureSection load='6sk4' size='340' side='right'caption='[[6sk4]], [[Resolution|resolution]] 1.55Å' scene=''> | | <StructureSection load='6sk4' size='340' side='right'caption='[[6sk4]], [[Resolution|resolution]] 1.55Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6sk4]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Deshy Deshy]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SK4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6SK4 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6sk4]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Desulfitobacterium_hafniense_Y51 Desulfitobacterium hafniense Y51]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6SK4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6SK4 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=THH:N-[4-({[(6S)-2-AMINO-4-HYDROXY-5-METHYL-5,6,7,8-TETRAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GLUTAMIC+ACID'>THH</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.55Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6sj8|6sj8]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=THH:N-[4-({[(6S)-2-AMINO-4-HYDROXY-5-METHYL-5,6,7,8-TETRAHYDROPTERIDIN-6-YL]METHYL}AMINO)BENZOYL]-L-GLUTAMIC+ACID'>THH</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">mtgA, DSY3157 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=138119 DESHY])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6sk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sk4 OCA], [https://pdbe.org/6sk4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6sk4 RCSB], [https://www.ebi.ac.uk/pdbsum/6sk4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6sk4 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6sk4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6sk4 OCA], [http://pdbe.org/6sk4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6sk4 RCSB], [http://www.ebi.ac.uk/pdbsum/6sk4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6sk4 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/MTGA_DESHY MTGA_DESHY]] Methyltransferase able to catalyze the transfer of a methyl group from methylcobalamin (methylCbl) to tetrahydrofolate (THF) in vitro, to generate methyl-THF and cob(I)alamin. In vivo, the methyl group probably comes from the adjacently encoded methylated corrinoid protein DSY3155. The methyl group may then be ultimately converted to carbon dioxide, and its oxidation would also provide reducing equivalents for anaerobic respiration. Thus, may function in the pathway that allows anaerobic methylotrophic growth of D.hafniense using glycine betaine.<ref>PMID:25313086</ref> | + | [https://www.uniprot.org/uniprot/MTGA_DESHY MTGA_DESHY] Methyltransferase able to catalyze the transfer of a methyl group from methylcobalamin (methylCbl) to tetrahydrofolate (THF) in vitro, to generate methyl-THF and cob(I)alamin. In vivo, the methyl group probably comes from the adjacently encoded methylated corrinoid protein DSY3155. The methyl group may then be ultimately converted to carbon dioxide, and its oxidation would also provide reducing equivalents for anaerobic respiration. Thus, may function in the pathway that allows anaerobic methylotrophic growth of D.hafniense using glycine betaine.<ref>PMID:25313086</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Deshy]] | + | [[Category: Desulfitobacterium hafniense Y51]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Badmann, T]] | + | [[Category: Badmann T]] |
| - | [[Category: Groll, M]] | + | [[Category: Groll M]] |
| - | [[Category: Anaerobic bacteria]]
| + | |
| - | [[Category: Cobalamin]]
| + | |
| - | [[Category: Glycine betaine metabolism]]
| + | |
| - | [[Category: Methyl transfer]]
| + | |
| - | [[Category: Tetrahydrofolate]]
| + | |
| - | [[Category: Transferase]]
| + | |
| Structural highlights
Function
MTGA_DESHY Methyltransferase able to catalyze the transfer of a methyl group from methylcobalamin (methylCbl) to tetrahydrofolate (THF) in vitro, to generate methyl-THF and cob(I)alamin. In vivo, the methyl group probably comes from the adjacently encoded methylated corrinoid protein DSY3155. The methyl group may then be ultimately converted to carbon dioxide, and its oxidation would also provide reducing equivalents for anaerobic respiration. Thus, may function in the pathway that allows anaerobic methylotrophic growth of D.hafniense using glycine betaine.[1]
Publication Abstract from PubMed
Enzymes orchestrating methylation between tetrahydrofolate (THF) and cobalamin (Cbl) are abundant among all domains of life. During energy production in Desulfitobacterium hafniense, MtgA catalyzes the methyl transfer from methylcobalamin (Cbl-CH3 ) to THF in the catabolism of glycine betaine (GB). Despite its lack of sequence identity with known structures, we could show that MtgA forms a homodimeric complex of two TIM barrels. Atomic crystallographic insights into the interplay of MtgA with THF as well as analysis of a trapped reaction intermediate (THF-CH3 )(+) reveal conformational rearrangements during the transfer reaction. Whereas residues for THF methylation are conserved, the binding mode for the THF glutamyl-p-aminobenzoate moiety (THF tail) is unique. Apart from snapshots of individual reaction steps of MtgA, structure-based mutagenesis combined with enzymatic activity assays allowed a mechanistic description of the methyl transfer between Cbl-CH3 and THF. Altogether, the THF-tail-binding motion observed in MtgA is unique compared to other THF methyltransferases and therefore contributes to the general understanding of THF-mediated methyl transfer.
Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution.,Badmann T, Groll M Chembiochem. 2019 Sep 13. doi: 10.1002/cbic.201900515. PMID:31518049[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Ticak T, Kountz DJ, Girosky KE, Krzycki JA, Ferguson DJ Jr. A nonpyrrolysine member of the widely distributed trimethylamine methyltransferase family is a glycine betaine methyltransferase. Proc Natl Acad Sci U S A. 2014 Oct 28;111(43):E4668-76. doi:, 10.1073/pnas.1409642111. Epub 2014 Oct 13. PMID:25313086 doi:http://dx.doi.org/10.1073/pnas.1409642111
- ↑ Badmann T, Groll M. Structures in Tetrahydrofolate Methylation in Desulfitobacterial Glycine Betaine Metabolism at Atomic Resolution. Chembiochem. 2019 Sep 13. doi: 10.1002/cbic.201900515. PMID:31518049 doi:http://dx.doi.org/10.1002/cbic.201900515
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