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| | <StructureSection load='6st8' size='340' side='right'caption='[[6st8]], [[Resolution|resolution]] 2.04Å' scene=''> | | <StructureSection load='6st8' size='340' side='right'caption='[[6st8]], [[Resolution|resolution]] 2.04Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6st8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Fraan Fraan]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6ST8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6st8]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Fragaria_x_ananassa Fragaria x ananassa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6ST8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6ST8 FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5amw|5amw]]</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FRAA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3747 FRAAN])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st8 OCA], [https://pdbe.org/6st8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6st8 RCSB], [https://www.ebi.ac.uk/pdbsum/6st8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6st8 ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6st8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6st8 OCA], [http://pdbe.org/6st8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6st8 RCSB], [http://www.ebi.ac.uk/pdbsum/6st8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6st8 ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/FRA12_FRAAN FRA12_FRAAN]] Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7]<ref>PMID:24133217</ref> | + | [https://www.uniprot.org/uniprot/FRA12_FRAAN FRA12_FRAAN] Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7]<ref>PMID:24133217</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fraan]] | |
| - | [[Category: Large Structures]] | |
| - | [[Category: Casanal, A]] | |
| - | [[Category: Dupeux, F]] | |
| - | [[Category: Kaczmarska, Z]] | |
| - | [[Category: Marquez, J A]] | |
| - | [[Category: Merchante, C]] | |
| - | [[Category: Orozco-Navarrete, B]] | |
| - | [[Category: Perales, A Diaz]] | |
| - | [[Category: Pott, D]] | |
| - | [[Category: Valpuesta, V]] | |
| - | [[Category: Allergen]] | |
| - | [[Category: Fra a 1]] | |
| | [[Category: Fragaria x ananassa]] | | [[Category: Fragaria x ananassa]] |
| - | [[Category: Fruit]] | + | [[Category: Large Structures]] |
| - | [[Category: Pr-10]] | + | [[Category: Casanal A]] |
| - | [[Category: Ripening]] | + | [[Category: Diaz Perales A]] |
| | + | [[Category: Dupeux F]] |
| | + | [[Category: Kaczmarska Z]] |
| | + | [[Category: Marquez JA]] |
| | + | [[Category: Merchante C]] |
| | + | [[Category: Orozco-Navarrete B]] |
| | + | [[Category: Pott D]] |
| | + | [[Category: Valpuesta V]] |
| Structural highlights
Function
FRA12_FRAAN Involved in the control of flavonoid biosynthesis in fruits, probably by binding directly to natural flavonoids (By similarity). Binds the natural flavonoid myricetin with affinities in the low micromolar range (PubMed:24133217).[UniProtKB:D0E0C7][1]
Publication Abstract from PubMed
Although strawberries are highly appreciated fruits, their intake can induce allergic reactions in atopic patients. These reactions can be due to the patient s previous sensitization to the major birch-pollen allergen Bet v 1, by which, IgE generated in response to Bet v 1 crossreacts with the structurally-related strawberry Fra a 1 protein family. Fra a 1.02 is the most expressed paralog in ripe strawberries and is highly allergenic. To better understand the molecular mechanisms regulating this allergic response, we have determined the three-dimensional structure of Fra a 1.02 and four site-directed mutants that were designed based on their positions in potential epitopes. Fra a 1.02 and mutants conform to the START fold. We show that the cross-reactivity of all the mutant variants to IgE from patients allergic to Bet v 1 was significantly reduced without altering the conserved structural fold, so that they could potentially be used as hypoallergenic Fra a 1 variants for the generation of vaccines against strawberry allergy in atopic patients.
STRUCTURAL BASIS FOR THE ALLERGENICITY OF Fra a 1.02 IN STRAWBERRY FRUITS.,Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Aranda M, Pott D, Diaz-Perales A, Casanal A, Marquez JA, Valpuesta V, Merchante C J Agric Food Chem. 2019 Nov 27. doi: 10.1021/acs.jafc.9b05714. PMID:31774998[2]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Casanal A, Zander U, Munoz C, Dupeux F, Luque I, Botella MA, Schwab W, Valpuesta V, Marquez JA. The Strawberry Pathogenesis-Related 10 (PR-10) Fra a proteins control flavonoid biosynthesis by binding to metabolic intermediates. J Biol Chem. 2013 Oct 16. PMID:24133217 doi:http://dx.doi.org/10.1074/jbc.M113.501528
- ↑ Orozco-Navarrete B, Kaczmarska Z, Dupeux F, Garrido-Aranda M, Pott D, Diaz-Perales A, Casanal A, Marquez JA, Valpuesta V, Merchante C. STRUCTURAL BASIS FOR THE ALLERGENICITY OF Fra a 1.02 IN STRAWBERRY FRUITS. J Agric Food Chem. 2019 Nov 27. doi: 10.1021/acs.jafc.9b05714. PMID:31774998 doi:http://dx.doi.org/10.1021/acs.jafc.9b05714
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