6tby

From Proteopedia

(Difference between revisions)

Current revision

Phycocyanobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB

Structural highlights

6tby is a 1 chain structure with sequence from Sorghum bicolor. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.8Å
Ligands:	, , ,
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

PHYB_SORBI Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion.[UniProtKB:P14713]

Publication Abstract from PubMed

Plant phytochromes are red/far-red photochromic photoreceptors that act as master regulators of development, controlling the expression of thousands of genes. Here, we describe the crystal structures of four plant phytochrome sensory modules, three at about 2 A resolution or better, including the first of an A-type phytochrome. Together with extensive spectral data, these structures provide detailed insight into the structure and function of plant phytochromes. In the Pr state, the substitution of phycocyanobilin and phytochromobilin cofactors has no structural effect, nor does the amino-terminal extension play a significant functional role. Our data suggest that the chromophore propionates and especially the phytochrome-specific domain tongue act differently in plant and prokaryotic phytochromes. We find that the photoproduct in period-ARNT-single-minded (PAS)-cGMP-specific phosphodiesterase-adenylyl cyclase-FhlA (GAF) bidomains might represent a novel intermediate between MetaRc and Pfr. We also discuss the possible role of a likely nuclear localization signal specific to and conserved in the phytochrome A lineage.

Structural insights into photoactivation and signalling in plant phytochromes.,Nagano S, Guan K, Shenkutie SM, Feiler C, Weiss M, Kraskov A, Buhrke D, Hildebrandt P, Hughes J Nat Plants. 2020 May 4. pii: 10.1038/s41477-020-0638-y. doi:, 10.1038/s41477-020-0638-y. PMID:32366982^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Nagano S, Guan K, Shenkutie SM, Feiler C, Weiss M, Kraskov A, Buhrke D, Hildebrandt P, Hughes J. Structural insights into photoactivation and signalling in plant phytochromes. Nat Plants. 2020 May 4. pii: 10.1038/s41477-020-0638-y. doi:, 10.1038/s41477-020-0638-y. PMID:32366982 doi:http://dx.doi.org/10.1038/s41477-020-0638-y

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6tby"

@@ Line 3: / Line 3: @@
 <StructureSection load='6tby' size='340' side='right'caption='[[6tby]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6tby]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Andropogon_sorghum Andropogon sorghum]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TBY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TBY FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6tby]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sorghum_bicolor Sorghum bicolor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TBY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TBY FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.8&#8491;</td></tr>
-<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PHYB, SORBI_3001G394400 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4558 Andropogon sorghum])</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BME:BETA-MERCAPTOETHANOL'>BME</scene>, <scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tby OCA], [http://pdbe.org/6tby PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tby RCSB], [http://www.ebi.ac.uk/pdbsum/6tby PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tby ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tby FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tby OCA], [https://pdbe.org/6tby PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tby RCSB], [https://www.ebi.ac.uk/pdbsum/6tby PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tby ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/Q6S527_SORBI Q6S527_SORBI]] Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region.[PIRNR:PIRNR000084]
+[https://www.uniprot.org/uniprot/PHYB_SORBI PHYB_SORBI] Regulatory photoreceptor which exists in two forms that are reversibly interconvertible by light: the Pr form that absorbs maximally in the red region of the spectrum and the Pfr form that absorbs maximally in the far-red region. Photoconversion of Pr to Pfr induces an array of morphogenic responses, whereas reconversion of Pfr to Pr cancels the induction of those responses. Pfr controls the expression of a number of nuclear genes including those encoding the small subunit of ribulose-bisphosphate carboxylase, chlorophyll A/B binding protein, protochlorophyllide reductase, rRNA, etc. It also controls the expression of its own gene(s) in a negative feedback fashion.[UniProtKB:P14713]
 <div style="background-color:#fffaf0;">
 == Publication Abstract from PubMed ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
-[[Category: Andropogon sorghum]]
 [[Category: Large Structures]]
-[[Category: Guan, K]]
+[[Category: Sorghum bicolor]]
-[[Category: Hughes, J E]]
+[[Category: Guan K]]
-[[Category: Nagano, S]]
+[[Category: Hughes JE]]
-[[Category: Shenkutie, S M]]
+[[Category: Nagano S]]
-[[Category: Phytochrome]]
+[[Category: Shenkutie SM]]
-[[Category: Plant protein]]

6tby

From Proteopedia

Current revision

Phycocyanobilin-adducted PAS-GAF bidomain of Sorghum bicolor phyB

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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