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| | <StructureSection load='6tic' size='340' side='right'caption='[[6tic]], [[Resolution|resolution]] 1.28Å' scene=''> | | <StructureSection load='6tic' size='340' side='right'caption='[[6tic]], [[Resolution|resolution]] 1.28Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6tic]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/A._niger A. niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TIC FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6tic]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_niger Aspergillus niger]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TIC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TIC FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BYN:hydroxylated+prenyl-FMN'>BYN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ND8:benzothiophene+2+carboxylic+acid'>ND8</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.281Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6tib|6tib]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BYN:hydroxylated+prenyl-FMN'>BYN</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=ND8:benzothiophene+2+carboxylic+acid'>ND8</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FDC1, ATCC64974_75990, CAN33_0036860 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5061 A. niger])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tic OCA], [https://pdbe.org/6tic PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tic RCSB], [https://www.ebi.ac.uk/pdbsum/6tic PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tic ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phenacrylate_decarboxylase Phenacrylate decarboxylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.1.1.102 4.1.1.102] </span></td></tr>
| + | |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tic FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tic OCA], [http://pdbe.org/6tic PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tic RCSB], [http://www.ebi.ac.uk/pdbsum/6tic PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tic ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/A0A3F3RNU4_ASPNG A0A3F3RNU4_ASPNG]] Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites.[HAMAP-Rule:MF_03196] | + | [https://www.uniprot.org/uniprot/A0A3F3RNU4_ASPNG A0A3F3RNU4_ASPNG] Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites.[HAMAP-Rule:MF_03196] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: A. niger]] | + | [[Category: Aspergillus niger]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Phenacrylate decarboxylase]]
| + | [[Category: Leys D]] |
| - | [[Category: Leys, D]] | + | |
| - | [[Category: Aromatic acid]]
| + | |
| - | [[Category: Ligase]]
| + | |
| - | [[Category: Prfmn]]
| + | |
| - | [[Category: Ubid]]
| + | |
| Structural highlights
Function
A0A3F3RNU4_ASPNG Catalyzes the reversible decarboxylation of aromatic carboxylic acids like ferulic acid, p-coumaric acid or cinnamic acid, producing the corresponding vinyl derivatives 4-vinylphenol, 4-vinylguaiacol, and styrene, respectively, which play the role of aroma metabolites.[HAMAP-Rule:MF_03196]
Publication Abstract from PubMed
The direct C-H carboxylation of aromatic compounds is an attractive route to the corresponding carboxylic acids, but remains challenging under mild conditions. It has been proposed that the first step in anaerobic microbial degradation of recalcitrant aromatic compounds is a UbiD-mediated carboxylation. In this study, we use the UbiD enzyme ferulic acid decarboxylase (Fdc) in combination with a carboxylic acid reductase to create aromatic degradation-inspired cascade reactions, leading to efficient functionalization of styrene through CO2 fixation. We reveal that rational structure-guided laboratory evolution can expand the substrate scope of Fdc, resulting in activity on a range of mono- and bicyclic aromatic compounds through a single mutation. Selected variants demonstrated 150-fold improvement in the conversion of coumarillic acid to benzofuran + CO2 and unlocked reactivity towards naphthoic acid. Our data demonstrate that UbiD-mediated C-H activation is a versatile tool for the transformation of aryl/alkene compounds and CO2 into commodity chemicals.
Enzymatic C-H activation of aromatic compounds through CO2 fixation.,Aleku GA, Saaret A, Bradshaw-Allen RT, Derrington SR, Titchiner GR, Gostimskaya I, Gahloth D, Parker DA, Hay S, Leys D Nat Chem Biol. 2020 Jul 27. pii: 10.1038/s41589-020-0603-0. doi:, 10.1038/s41589-020-0603-0. PMID:32719558[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Aleku GA, Saaret A, Bradshaw-Allen RT, Derrington SR, Titchiner GR, Gostimskaya I, Gahloth D, Parker DA, Hay S, Leys D. Enzymatic C-H activation of aromatic compounds through CO2 fixation. Nat Chem Biol. 2020 Jul 27. pii: 10.1038/s41589-020-0603-0. doi:, 10.1038/s41589-020-0603-0. PMID:32719558 doi:http://dx.doi.org/10.1038/s41589-020-0603-0
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