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| | <StructureSection load='6yfe' size='340' side='right'caption='[[6yfe]], [[Resolution|resolution]] 3.79Å' scene=''> | | <StructureSection load='6yfe' size='340' side='right'caption='[[6yfe]], [[Resolution|resolution]] 3.79Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6yfe]] is a 90 chain structure with sequence from [http://en.wikipedia.org/wiki/Beihai_levi-like_virus_19 Beihai levi-like virus 19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFE OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6YFE FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6yfe]] is a 90 chain structure with sequence from [https://en.wikipedia.org/wiki/Beihai_levi-like_virus_19 Beihai levi-like virus 19]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6YFE OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6YFE FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.794Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6yf7|6yf7]], [[6yf9|6yf9]], [[6yfa|6yfa]], [[6yfb|6yfb]], [[6yfc|6yfc]], [[6yfd|6yfd]], [[6yff|6yff]], [[6yfg|6yfg]], [[6yfh|6yfh]], [[6yfi|6yfi]], [[6yfj|6yfj]], [[6yfk|6yfk]], [[6yfl|6yfl]], [[6yfm|6yfm]], [[6yfn|6yfn]], [[6yfo|6yfo]], [[6yfp|6yfp]], [[6yfq|6yfq]], [[6yfr|6yfr]], [[6yfs|6yfs]], [[6yft|6yft]], [[6yfu|6yfu]]</div></td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6yfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfe OCA], [http://pdbe.org/6yfe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6yfe RCSB], [http://www.ebi.ac.uk/pdbsum/6yfe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfe ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6yfe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6yfe OCA], [https://pdbe.org/6yfe PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6yfe RCSB], [https://www.ebi.ac.uk/pdbsum/6yfe PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6yfe ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
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| | [[Category: Beihai levi-like virus 19]] | | [[Category: Beihai levi-like virus 19]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Kalnins, G]] | + | [[Category: Kalnins G]] |
| - | [[Category: Lieknina, I]] | + | [[Category: Lieknina I]] |
| - | [[Category: Rumnieks, J]] | + | [[Category: Rumnieks J]] |
| - | [[Category: Sisovs, M]] | + | [[Category: Sisovs M]] |
| - | [[Category: Tars, K]] | + | [[Category: Tars K]] |
| - | [[Category: Structural protein]]
| + | |
| - | [[Category: Virus]]
| + | |
| - | [[Category: Virus like particle]]
| + | |
| - | [[Category: Virus-like particle]]
| + | |
| Structural highlights
Publication Abstract from PubMed
The single-stranded RNA (ssRNA) bacteriophages are among the simplest known viruses with small genomes and exceptionally high mutation rates. The number of ssRNA phage isolates has remained very low, but recent metagenomic studies have uncovered an immense variety of distinct uncultured ssRNA phages. The coat proteins (CPs) in these genomes are particularly diverse, with notable variation in length and often no recognizable similarity to previously known viruses. We recombinantly expressed metagenome-derived ssRNA phage CPs to produce virus-like particles and determined the three-dimensional structure of 22 previously uncharacterized ssRNA phage capsids covering nine distinct CP types. The structures revealed substantial deviations from the previously known ssRNA phage CP fold, uncovered an unusual prolate particle shape, and revealed a previously unseen dsRNA binding mode. These data expand our knowledge of the evolution of viral structural proteins and are of relevance for applications such as ssRNA phage-based vaccine design.
Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes.,Rumnieks J, Lieknina I, Kalnins G, Sisovs M, Akopjana I, Bogans J, Tars K Sci Adv. 2020 Sep 2;6(36). pii: 6/36/eabc0023. doi: 10.1126/sciadv.abc0023. Print, 2020 Sep. PMID:32917600[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Rumnieks J, Lieknina I, Kalnins G, Sisovs M, Akopjana I, Bogans J, Tars K. Three-dimensional structure of 22 uncultured ssRNA bacteriophages: Flexibility of the coat protein fold and variations in particle shapes. Sci Adv. 2020 Sep 2;6(36). pii: 6/36/eabc0023. doi: 10.1126/sciadv.abc0023. Print, 2020 Sep. PMID:32917600 doi:http://dx.doi.org/10.1126/sciadv.abc0023
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