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| | <StructureSection load='6z0o' size='340' side='right'caption='[[6z0o]], [[Resolution|resolution]] 2.60Å' scene=''> | | <StructureSection load='6z0o' size='340' side='right'caption='[[6z0o]], [[Resolution|resolution]] 2.60Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6z0o]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Cchfv Cchfv] and [http://en.wikipedia.org/wiki/Synthetic_construct_sequences Synthetic construct sequences]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z0O OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6Z0O FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6z0o]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Crimean-Congo_hemorrhagic_fever_orthonairovirus Crimean-Congo hemorrhagic fever orthonairovirus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6Z0O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6Z0O FirstGlance]. <br> |
| - | </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[4akl|4akl]]</div></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.5974Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Np ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1980519 CCHFV])</td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6z0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z0o OCA], [https://pdbe.org/6z0o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6z0o RCSB], [https://www.ebi.ac.uk/pdbsum/6z0o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6z0o ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6z0o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6z0o OCA], [http://pdbe.org/6z0o PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6z0o RCSB], [http://www.ebi.ac.uk/pdbsum/6z0o PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6z0o ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | + | == Function == |
| | + | [https://www.uniprot.org/uniprot/Q70UR4_9VIRU Q70UR4_9VIRU] |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Cchfv]] | + | [[Category: Crimean-Congo hemorrhagic fever orthonairovirus]] |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Synthetic construct sequences]] | + | [[Category: Synthetic construct]] |
| - | [[Category: Alvarez-Rodriguez, B]] | + | [[Category: Alvarez-Rodriguez B]] |
| - | [[Category: Barr, J N]] | + | [[Category: Barr JN]] |
| - | [[Category: Edwards, T A]] | + | [[Category: Edwards TA]] |
| - | [[Category: Tiede, C]] | + | [[Category: Tiede C]] |
| - | [[Category: Tomlinson, D]] | + | [[Category: Tomlinson D]] |
| - | [[Category: Trinh, C]] | + | [[Category: Trinh C]] |
| - | [[Category: Affimer-np]]
| + | |
| - | [[Category: Anti-viral]]
| + | |
| - | [[Category: Complex]]
| + | |
| - | [[Category: Crimean-congo hemorrhagic fever virus nucleoprotein]]
| + | |
| - | [[Category: High-affinity]]
| + | |
| - | [[Category: Rna-binding]]
| + | |
| - | [[Category: Viral protein]]
| + | |
| Structural highlights
Function
Q70UR4_9VIRU
Publication Abstract from PubMed
Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV) is one of the most widespread medically important arboviruses, causing human infections that result in mortality rates of up to 60%. We describe the selection of a high-affinity small protein (Affimer-NP) that binds specifically to the nucleoprotein (NP) of CCHFV. We demonstrate the interference of Affimer-NP in the RNA-binding function of CCHFV NP using fluorescence anisotropy, and its inhibitory effects on CCHFV gene expression in mammalian cells using a mini-genome system. Solution of the crystallographic structure of the complex formed by these two molecules at 2.84 A resolution revealed the structural basis for this interference, with the Affimer-NP binding site positioned at the critical NP oligomerization interface. Finally, we validate the in vitro application of Affimer-NP for the development of enzyme-linked immunosorbent and lateral flow assays, presenting the first published point-of-care format test able to detect recombinant CCHFV NP in spiked human and animal sera.
Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests.,Alvarez-Rodriguez B, Tiede C, Hoste ACR, Surtees RA, Trinh CH, Slack GS, Chamberlain J, Hewson R, Fresco A, Sastre P, Tomlinson DC, Millner PA, Edwards TA, Barr JN PLoS Negl Trop Dis. 2020 Jun 3;14(6):e0008364. doi: 10.1371/journal.pntd.0008364., eCollection 2020 Jun. PMID:32492018[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Alvarez-Rodriguez B, Tiede C, Hoste ACR, Surtees RA, Trinh CH, Slack GS, Chamberlain J, Hewson R, Fresco A, Sastre P, Tomlinson DC, Millner PA, Edwards TA, Barr JN. Characterization and applications of a Crimean-Congo hemorrhagic fever virus nucleoprotein-specific Affimer: Inhibitory effects in viral replication and development of colorimetric diagnostic tests. PLoS Negl Trop Dis. 2020 Jun 3;14(6):e0008364. doi: 10.1371/journal.pntd.0008364., eCollection 2020 Jun. PMID:32492018 doi:http://dx.doi.org/10.1371/journal.pntd.0008364
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