3j1x
From Proteopedia
(Difference between revisions)
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<SX load='3j1x' size='340' side='right' viewer='molstar' caption='[[3j1x]], [[Resolution|resolution]] 11.70Å' scene=''> | <SX load='3j1x' size='340' side='right' viewer='molstar' caption='[[3j1x]], [[Resolution|resolution]] 11.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[3j1x]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp. | + | <table><tr><td colspan='2'>[[3j1x]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Salmonella_enterica_subsp._enterica_serovar_Typhimurium Salmonella enterica subsp. enterica serovar Typhimurium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3J1X OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3J1X FirstGlance]. <br> |
| - | </td></tr><tr id=' | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 11.7Å</td></tr> |
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1x OCA], [https://pdbe.org/3j1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j1x RCSB], [https://www.ebi.ac.uk/pdbsum/3j1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1x ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3j1x FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3j1x OCA], [https://pdbe.org/3j1x PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3j1x RCSB], [https://www.ebi.ac.uk/pdbsum/3j1x PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3j1x ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
| - | + | [https://www.uniprot.org/uniprot/PRGH_SALTY PRGH_SALTY] Required for invasion of epithelial cells. | |
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| - | + | The T3SS injectisome is a syringe-shaped macromolecular assembly found in pathogenic Gram-negative bacteria that allows for the direct delivery of virulence effectors into host cells. It is composed of a "basal body", a lock-nut structure spanning both bacterial membranes, and a "needle" that protrudes away from the bacterial surface. A hollow channel spans throughout the apparatus, permitting the translocation of effector proteins from the bacterial cytosol to the host plasma membrane. The basal body is composed largely of three membrane-embedded proteins that form oligomerized concentric rings. Here, we report the crystal structures of three domains of the prototypical Salmonella SPI-1 basal body, and use a new approach incorporating symmetric flexible backbone docking and EM data to produce a model for their oligomeric assembly. The obtained models, validated by biochemical and in vivo assays, reveal the molecular details of the interactions driving basal body assembly, and notably demonstrate a conserved oligomerization mechanism. | |
| - | + | A Refined Model of the Prototypical Salmonella SPI-1 T3SS Basal Body Reveals the Molecular Basis for Its Assembly.,Bergeron JR, Worrall LJ, Sgourakis NG, Dimaio F, Pfuetzner RA, Felise HB, Vuckovic M, Yu AC, Miller SI, Baker D, Strynadka NC PLoS Pathog. 2013 Apr;9(4):e1003307. doi: 10.1371/journal.ppat.1003307. Epub 2013, Apr 25. PMID:23633951<ref>PMID:23633951</ref> | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
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</SX> | </SX> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: Salmonella enterica subsp. enterica serovar | + | [[Category: Salmonella enterica subsp. enterica serovar Typhimurium]] |
| - | [[Category: Baker | + | [[Category: Baker D]] |
| - | [[Category: Bergeron | + | [[Category: Bergeron JRC]] |
| - | [[Category: Sgourakis | + | [[Category: Sgourakis NG]] |
| - | [[Category: Strynadka | + | [[Category: Strynadka NCJ]] |
| - | [[Category: Worrall | + | [[Category: Worrall LJ]] |
| - | + | ||
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Revision as of 14:13, 24 January 2024
A refined model of the prototypical Salmonella typhimurium T3SS basal body reveals the molecular basis for its assembly
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