1pt2

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[[Image:1pt2.gif|left|200px]]
[[Image:1pt2.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1pt2 |SIZE=350|CAPTION= <scene name='initialview01'>1pt2</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_1pt2", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SUC:SUCROSE'>SUC</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Levansucrase Levansucrase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.1.10 2.4.1.10] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= SACB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 Bacillus subtilis])
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-->
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|DOMAIN=
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{{STRUCTURE_1pt2| PDB=1pt2 | SCENE= }}
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|RELATEDENTRY=[[1oyg|1OYG]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pt2 OCA], [http://www.ebi.ac.uk/pdbsum/1pt2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pt2 RCSB]</span>
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}}
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'''Crystal structure of levansucrase (E342A) complexed with sucrose'''
'''Crystal structure of levansucrase (E342A) complexed with sucrose'''
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[[Category: Futterer, K.]]
[[Category: Futterer, K.]]
[[Category: Meng, G.]]
[[Category: Meng, G.]]
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[[Category: beta-propeller]]
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[[Category: Beta-propeller]]
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[[Category: glycoside hydrolase]]
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[[Category: Glycoside hydrolase]]
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[[Category: levan]]
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[[Category: Levan]]
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[[Category: levansucrase]]
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[[Category: Levansucrase]]
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[[Category: sucrose]]
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[[Category: Sucrose]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:27:22 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:09 2008''
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Revision as of 02:27, 3 May 2008

Image:1pt2.gif

Template:STRUCTURE 1pt2

Crystal structure of levansucrase (E342A) complexed with sucrose


Overview

Many bacteria and about 40,000 plant species form primary carbohydrate reserves based on fructan; these polymers of beta-D-fructofuranose are thought to confer tolerance to drought and frost in plants. Microbial fructan, the beta(2,6)-linked levan, is synthesized directly from sucrose by levansucrase, which is able to catalyze both sucrose hydrolysis and levan polymerization. The crystal structure of Bacillus subtilis levansucrase, determined to a resolution of 1.5 A, shows a rare five-fold beta-propeller topology with a deep, negatively charged central pocket. Arg360, a residue essential for polymerase activity, lies in a solvent-exposed site adjacent to the central pocket. Mutagenesis data and the sucrose-bound structure of inactive levansucrase E342A, at a resolution of 2.1 A, strongly suggest that three conserved acidic side chains in the central pocket are critical for catalysis, and presumably function as nucleophile (Asp86) and general acid (Glu342), or stabilize the transition state (Asp247).

About this Structure

1PT2 is a Single protein structure of sequence from Bacillus subtilis. Full crystallographic information is available from OCA.

Reference

Structural framework of fructosyl transfer in Bacillus subtilis levansucrase., Meng G, Futterer K, Nat Struct Biol. 2003 Nov;10(11):935-41. Epub 2003 Sep 28. PMID:14517548 Page seeded by OCA on Sat May 3 05:27:22 2008

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