1pt3

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[[Image:1pt3.gif|left|200px]]
[[Image:1pt3.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1pt3 |SIZE=350|CAPTION= <scene name='initialview01'>1pt3</scene>, resolution 2.50&Aring;
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The line below this paragraph, containing "STRUCTURE_1pt3", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= COLE7 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
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-->
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|DOMAIN=
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{{STRUCTURE_1pt3| PDB=1pt3 | SCENE= }}
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|RELATEDENTRY=[[7cei|7CEI]], [[1m08|1M08]], [[1mz8|1MZ8]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pt3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pt3 OCA], [http://www.ebi.ac.uk/pdbsum/1pt3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pt3 RCSB]</span>
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}}
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'''Crystal structures of nuclease-ColE7 complexed with octamer DNA'''
'''Crystal structures of nuclease-ColE7 complexed with octamer DNA'''
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[[Category: Ku, W Y.]]
[[Category: Ku, W Y.]]
[[Category: Yuan, H S.]]
[[Category: Yuan, H S.]]
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[[Category: colicin]]
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[[Category: Colicin]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: hnh motif]]
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[[Category: Hnh motif]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:27:25 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:12 2008''
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Revision as of 02:27, 3 May 2008

Image:1pt3.gif

Template:STRUCTURE 1pt3

Crystal structures of nuclease-ColE7 complexed with octamer DNA


Overview

The bacterial toxin ColE7 bears an HNH motif which has been identified in hundreds of prokaryotic and eukaryotic endonucleases, involved in DNA homing, restriction, repair, or chromosome degradation. The crystal structure of the nuclease domain of ColE7 in complex with a duplex DNA has been determined at 2.5 A resolution. The HNH motif is bound at the minor groove primarily to DNA phosphate groups at and beyond the 3' side of the scissile phosphate, with little interaction with ribose groups and bases. This result provides a structural basis for sugar- and sequence-independent DNA recognition and the inhibition mechanism by inhibitor Im7, which blocks the substrate binding site but not the active site. Structural comparison shows that two families of endonucleases bind and bend DNA in a similar way to that of the HNH ColE7, indicating that endonucleases containing a "betabetaalpha-metal" fold of active site possess a universal mode for protein-DNA interactions.

About this Structure

1PT3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

DNA binding and degradation by the HNH protein ColE7., Hsia KC, Chak KF, Liang PH, Cheng YS, Ku WY, Yuan HS, Structure. 2004 Feb;12(2):205-14. PMID:14962381 Page seeded by OCA on Sat May 3 05:27:25 2008

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