1pts

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[[Image:1pts.gif|left|200px]]
[[Image:1pts.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_1pts", creates the "Structure Box" on the page.
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|LIGAND= <scene name='pdbligand=PHR:3-(1,3,7-TRIHYDRO-9-D-RIBITYL-2,6,8-PURINETRIONE-7-YL+)+HEXANE+1-PHOSPHATE'>PHR</scene>
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pts OCA], [http://www.ebi.ac.uk/pdbsum/1pts PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pts RCSB]</span>
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'''CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN'''
'''CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN'''
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[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
[[Category: Group, Dupont Protein Crystallography.]]
[[Category: Group, Dupont Protein Crystallography.]]
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[[Category: glycoprotein]]
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[[Category: Glycoprotein]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:28:33 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:26 2008''
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Revision as of 02:28, 3 May 2008

Image:1pts.gif

Template:STRUCTURE 1pts

CRYSTAL STRUCTURE AND LIGAND BINDING STUDIES OF A SCREENED PEPTIDE COMPLEXED WITH STREPTAVIDIN


Overview

The thermodynamic binding parameters and crystal structure for streptavidin-peptide complexes where the peptide sequences were obtained by random screening methods are reported. The affinities between streptavidin and two heptapeptides were determined by titrating calorimetric methods [Phe-Ser-His-Pro-Gln-Asn-Thr, Ka = 7944 (+/- 224) M-1, delta G degrees = -5.32 (+/- 0.01) kcal/mol, and delta H degrees = -19.34 (+/- 0.48) kcal/mol; His-Asp-His-Pro-Gln-Asn-Leu, Ka = 3542 (+/- 146) M-1, delta G degrees = -4.84 (+/- 0.03) kcal/mol, and delta H degrees = -19.00 (+/- 0.64) kcal/mol]. The crystal structure of streptavidin complexed with one of these peptides has been determined at 2.0-A resolution. The peptide (Phe-Ser-His-Pro-Gln-Asn-Thr) binds in a turn conformation with the histidine, proline, and glutamine side chains oriented inward at the biotin-binding site. A water molecule is immobilized between the histidine and glutamine side chains of the peptide and an aspartic acid side chain of the protein. Although some of the residues that participate in binding biotin also interact with the screened peptide, the peptide adopts an alternate method of utilizing binding determinants in the biotin-binding site of streptavidin.

About this Structure

1PTS is a Single protein structure of sequence from Streptomyces avidinii. Full crystallographic information is available from OCA.

Reference

Crystal structure and ligand-binding studies of a screened peptide complexed with streptavidin., Weber PC, Pantoliano MW, Thompson LD, Biochemistry. 1992 Oct 6;31(39):9350-4. PMID:1390720 Page seeded by OCA on Sat May 3 05:28:33 2008

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