1pud

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[[Image:1pud.gif|left|200px]]
[[Image:1pud.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 1pud |SIZE=350|CAPTION= <scene name='initialview01'>1pud</scene>, resolution 1.85&Aring;
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The line below this paragraph, containing "STRUCTURE_1pud", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Queuine_tRNA-ribosyltransferase Queuine tRNA-ribosyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.4.2.29 2.4.2.29] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= TGT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=542 Zymomonas mobilis])
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-->
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|DOMAIN=
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{{STRUCTURE_1pud| PDB=1pud | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1pud FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1pud OCA], [http://www.ebi.ac.uk/pdbsum/1pud PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1pud RCSB]</span>
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}}
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'''TRNA-GUANINE TRANSGLYCOSYLASE'''
'''TRNA-GUANINE TRANSGLYCOSYLASE'''
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[[Category: Romier, C.]]
[[Category: Romier, C.]]
[[Category: Suck, D.]]
[[Category: Suck, D.]]
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[[Category: glycosyltransferase]]
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[[Category: Glycosyltransferase]]
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[[Category: transferase]]
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[[Category: Transferase]]
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[[Category: trna-modifying enzyme]]
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[[Category: Trna-modifying enzyme]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sat May 3 05:29:37 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 23:04:40 2008''
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Revision as of 02:29, 3 May 2008

Image:1pud.gif

Template:STRUCTURE 1pud

TRNA-GUANINE TRANSGLYCOSYLASE


Overview

tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with the queuine (.)precursor 7-aminomethyl-7-deazaguanine (preQ1). The crystal structure of TGT from Zymomonas mobilis was solved by multiple isomorphous replacement and refined to a crystallographic R-factor of 19% at 1.85 angstrom resolution. The structure consists of an irregular (beta/alpha)8-barrel with a tightly attached C-terminal zinc-containing subdomain. The packing of the subdomain against the barrel is mediated by an alpha-helix, located close to the C-terminus, which displaces the eighth helix of the barrel. The structure of TGT in complex with preQ1 suggests a binding mode for tRNA where the phosphate backbone interacts with the zinc subdomain and the U33G34U35 sequence is recognized by the barrel. This model for tRNA binding is consistent with a base exchange mechanism involving a covalent tRNA-enzyme intermediate. This structure is the first example of a (beta/alpha)-barrel protein interacting specifically with a nucleic acid.

About this Structure

1PUD is a Single protein structure of sequence from Zymomonas mobilis. Full crystallographic information is available from OCA.

Reference

Crystal structure of tRNA-guanine transglycosylase: RNA modification by base exchange., Romier C, Reuter K, Suck D, Ficner R, EMBO J. 1996 Jun 3;15(11):2850-7. PMID:8654383 Page seeded by OCA on Sat May 3 05:29:37 2008

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OCA

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