7aa8

From Proteopedia

(Difference between revisions)

Current revision

Structure of SCOC LIR bound to GABARAP

Structural highlights

7aa8 is a 1 chain structure with sequence from Homo sapiens. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:	X-ray diffraction, Resolution 1.25Å
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GBRAP_HUMAN May play a role in intracellular transport of GABA(A) receptors and its interaction with the cytoskeleton. Involved in apoptosis. Involved in autophagy (By similarity).^[1]

Publication Abstract from PubMed

Autophagy is a highly conserved degradative pathway, essential for cellular homeostasis and implicated in diseases including cancer and neurodegeneration. Autophagy-related 8 (ATG8) proteins play a central role in autophagosome formation and selective delivery of cytoplasmic cargo to lysosomes by recruiting autophagy adaptors and receptors. The LC3-interacting region (LIR) docking site (LDS) of ATG8 proteins binds to LIR motifs present in autophagy adaptors and receptors. LIR-ATG8 interactions can be highly selective for specific mammalian ATG8 family members (LC3A-C, GABARAP, and GABARAPL1-2) and how this specificity is generated and regulated is incompletely understood. We have identified a LIR motif in the Golgi protein SCOC (short coiled-coil protein) exhibiting strong binding to GABARAP, GABARAPL1, LC3A and LC3C. The residues within and surrounding the core LIR motif of the SCOC LIR domain were phosphorylated by autophagy-related kinases (ULK1-3, TBK1) increasing specifically LC3 family binding. More distant flanking residues also contributed to ATG8 binding. Loss of these residues was compensated by phosphorylation of serine residues immediately adjacent to the core LIR motif, indicating that the interactions of the flanking LIR regions with the LDS are important and highly dynamic. Our comprehensive structural, biophysical and biochemical analyses support and provide novel mechanistic insights into how phosphorylation of LIR domain residues regulates the affinity and binding specificity of ATG8 proteins towards autophagy adaptors and receptors.

Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity.,Wirth M, Mouilleron S, Zhang W, Sjottem E, Princely Abudu Y, Jain A, Lauritz Olsvik H, Bruun JA, Razi M, Jefferies HBJ, Lee R, Joshi D, O'Reilly N, Johansen T, Tooze SA J Mol Biol. 2021 Jun 25;433(13):166987. doi: 10.1016/j.jmb.2021.166987. Epub 2021 , Apr 24. PMID:33845085^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Lee JH, Rho SB, Chun T. GABAA receptor-associated protein (GABARAP) induces apoptosis by interacting with DEAD (Asp-Glu-Ala-Asp/His) box polypeptide 47 (DDX 47). Biotechnol Lett. 2005 May;27(9):623-8. PMID:15977068 doi:http://dx.doi.org/10.1007/s10529-005-3628-2
↑ Wirth M, Mouilleron S, Zhang W, Sjottem E, Princely Abudu Y, Jain A, Lauritz Olsvik H, Bruun JA, Razi M, Jefferies HBJ, Lee R, Joshi D, O'Reilly N, Johansen T, Tooze SA. Phosphorylation of the LIR Domain of SCOC Modulates ATG8 Binding Affinity and Specificity. J Mol Biol. 2021 Jun 25;433(13):166987. doi: 10.1016/j.jmb.2021.166987. Epub 2021 , Apr 24. PMID:33845085 doi:http://dx.doi.org/10.1016/j.jmb.2021.166987

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/7aa8"

@@ Line 4: / Line 4: @@
 == Structural highlights ==
 <table><tr><td colspan='2'>[[7aa8]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7AA8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7AA8 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aa8 OCA], [https://pdbe.org/7aa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aa8 RCSB], [https://www.ebi.ac.uk/pdbsum/7aa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aa8 ProSAT]</span></td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.25&#8491;</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7aa8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7aa8 OCA], [https://pdbe.org/7aa8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7aa8 RCSB], [https://www.ebi.ac.uk/pdbsum/7aa8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7aa8 ProSAT]</span></td></tr>
 </table>
 == Function ==

7aa8

From Proteopedia

Current revision

Structure of SCOC LIR bound to GABARAP

Structural highlights

Function

Publication Abstract from PubMed

References

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