7atl
From Proteopedia
(Difference between revisions)
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==EstCE1, a hydrolase with promiscuous acyltransferase activity== | ==EstCE1, a hydrolase with promiscuous acyltransferase activity== | ||
| - | <StructureSection load='7atl' size='340' side='right'caption='[[7atl]]' scene=''> | + | <StructureSection load='7atl' size='340' side='right'caption='[[7atl]], [[Resolution|resolution]] 2.48Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ATL OCA]. For a <b>guided tour on the structure components</b> use [ | + | <table><tr><td colspan='2'>[[7atl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultured_bacterium_pCosCE1 Uncultured bacterium pCosCE1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7ATL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7ATL FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.478Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7atl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7atl OCA], [https://pdbe.org/7atl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7atl RCSB], [https://www.ebi.ac.uk/pdbsum/7atl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7atl ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/Q1I192_9BACT Q1I192_9BACT] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Promiscuous acyltransferase activity is the ability of certain hydrolases to preferentially catalyze acyl transfer over hydrolysis, even in bulk water. However, poor enantioselectivity, low transfer efficiency, significant product hydrolysis, and limited substrate scope represent considerable drawbacks for their application. By activity-based screening of several hydrolases, we identified the family VIII carboxylesterase, EstCE1, as an unprecedentedly efficient acyltransferase. EstCE1 catalyzes the irreversible amidation and carbamoylation of amines in water, which enabled the synthesis of the drug moclobemide from methyl 4-chlorobenzoate and 4-(2-aminoethyl)morpholine (~20% conversion). We solved the crystal structure of EstCE1 and detailed structure-function analysis revealed a three-amino acid motif important for promiscuous acyltransferase activity. Introducing this motif into an esterase without acetyltransferase activity transformed a 'hydrolase' into an 'acyltransferase'. | ||
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| + | Discovery and Design of Family VIII Carboxylesterases as Highly Efficient Acyltransferases.,Muller H, Godehard SP, Palm GJ, Berndt L, Badenhorst CPS, Becker AK, Lammers M, Bornscheuer U Angew Chem Int Ed Engl. 2020 Nov 3. doi: 10.1002/anie.202014169. PMID:33140887<ref>PMID:33140887</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7atl" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Uncultured bacterium pCosCE1]] | ||
[[Category: Berndt L]] | [[Category: Berndt L]] | ||
[[Category: Lammers M]] | [[Category: Lammers M]] | ||
[[Category: Palm GJ]] | [[Category: Palm GJ]] | ||
Current revision
EstCE1, a hydrolase with promiscuous acyltransferase activity
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