7bba
From Proteopedia
(Difference between revisions)
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==Structure of the TagL peptidoglycan binding domain from EAEC T6SS== | ==Structure of the TagL peptidoglycan binding domain from EAEC T6SS== | ||
| - | <StructureSection load='7bba' size='340' side='right'caption='[[7bba]]' scene=''> | + | <StructureSection load='7bba' size='340' side='right'caption='[[7bba]], [[Resolution|resolution]] 2.43Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5m3g 5m3g]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBA FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bba]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=5m3g 5m3g]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BBA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BBA FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bba OCA], [https://pdbe.org/7bba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bba RCSB], [https://www.ebi.ac.uk/pdbsum/7bba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bba ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.43Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bba FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bba OCA], [https://pdbe.org/7bba PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bba RCSB], [https://www.ebi.ac.uk/pdbsum/7bba PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bba ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/D3GUV9_ECO44 D3GUV9_ECO44] | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The type VI secretion system (T6SS) is a widespread mechanism of protein delivery into target cells, present in more than a quarter of all sequenced Gram-negative bacteria. The T6SS constitutes an important virulence factor, as it is responsible for targeting effectors in both prokaryotic and eukaryotic cells. The T6SS comprises a tail structure tethered to the cell envelope via a trans-envelope complex. In most T6SS, the membrane complex is anchored to the cell wall by the TagL accessory protein. In this study, we report the first crystal structure of a peptidoglycan-binding domain of TagL. The fold is conserved with members of the OmpA/Pal/MotB family, and more importantly, the peptidoglycan binding site is conserved. This structure further exemplifies how proteins involved in anchoring to the cell wall for different cellular functions rely on an interaction network with peptidoglycan strictly conserved. | ||
| + | |||
| + | Anchoring the T6SS to the cell wall: Crystal structure of the peptidoglycan binding domain of the TagL accessory protein.,Nguyen VS, Spinelli S, Cascales E, Roussel A, Cambillau C, Leone P PLoS One. 2021 Jul 2;16(7):e0254232. doi: 10.1371/journal.pone.0254232., eCollection 2021. PMID:34214145<ref>PMID:34214145</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bba" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Escherichia coli]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
[[Category: Cambillau C]] | [[Category: Cambillau C]] | ||
[[Category: Leone P]] | [[Category: Leone P]] | ||
[[Category: Nguyen VS]] | [[Category: Nguyen VS]] | ||
Current revision
Structure of the TagL peptidoglycan binding domain from EAEC T6SS
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