7bnx
From Proteopedia
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==Archeal holliday junction resolvase from Thermus thermophilus phage 15-6== | ==Archeal holliday junction resolvase from Thermus thermophilus phage 15-6== | ||
| - | <StructureSection load='7bnx' size='340' side='right'caption='[[7bnx]]' scene=''> | + | <StructureSection load='7bnx' size='340' side='right'caption='[[7bnx]], [[Resolution|resolution]] 2.55Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BNX FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7bnx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Unclassified_bacterial_viruses Unclassified bacterial viruses]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7BNX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7BNX FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bnx OCA], [https://pdbe.org/7bnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bnx RCSB], [https://www.ebi.ac.uk/pdbsum/7bnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bnx ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.551Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7bnx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7bnx OCA], [https://pdbe.org/7bnx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7bnx RCSB], [https://www.ebi.ac.uk/pdbsum/7bnx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7bnx ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | This study describes the production, characterization and structure determination of a novel Holliday junction-resolving enzyme. The enzyme, termed Hjc_15-6, is encoded in the genome of phage Tth15-6, which infects Thermus thermophilus. Hjc_15-6 was heterologously produced in Escherichia coli and high yields of soluble and biologically active recombinant enzyme were obtained in both complex and defined media. Amino-acid sequence and structure comparison suggested that the enzyme belongs to a group of enzymes classified as archaeal Holliday junction-resolving enzymes, which are typically divalent metal ion-binding dimers that are able to cleave X-shaped dsDNA-Holliday junctions (Hjs). The crystal structure of Hjc_15-6 was determined to 2.5 A resolution using the selenomethionine single-wavelength anomalous dispersion method. To our knowledge, this is the first crystal structure of an Hj-resolving enzyme originating from a bacteriophage that can be classified as an archaeal type of Hj-resolving enzyme. As such, it represents a new fold for Hj-resolving enzymes from phages. Characterization of the structure of Hjc_15-6 suggests that it may form a dimer, or even a homodimer of dimers, and activity studies show endonuclease activity towards Hjs. Furthermore, based on sequence analysis it is proposed that Hjc_15-6 has a three-part catalytic motif corresponding to E-SD-EVK, and this motif may be common among other Hj-resolving enzymes originating from thermophilic bacteriophages. | ||
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| + | Crystal structure and initial characterization of a novel archaeal-like Holliday junction-resolving enzyme from Thermus thermophilus phage Tth15-6.,Ahlqvist J, Linares-Pasten JA, Hakansson M, Jasilionis A, Kwiatkowska-Semrau K, Friethjonsson OH, Kaczorowska AK, Dabrowski S, AEvarsson A, Hreggviethsson GO, Al-Karadaghi S, Kaczorowski T, Nordberg Karlsson E Acta Crystallogr D Struct Biol. 2022 Feb 1;78(Pt 2):212-227. doi:, 10.1107/S2059798321012298. Epub 2022 Jan 24. PMID:35102887<ref>PMID:35102887</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7bnx" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[Resolvase 3D structures|Resolvase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| + | [[Category: Unclassified bacterial viruses]] | ||
[[Category: Ahlqvist J]] | [[Category: Ahlqvist J]] | ||
[[Category: Al-Karadaghi S]] | [[Category: Al-Karadaghi S]] | ||
Current revision
Archeal holliday junction resolvase from Thermus thermophilus phage 15-6
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