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| | <StructureSection load='7ndh' size='340' side='right'caption='[[7ndh]], [[Resolution|resolution]] 1.94Å' scene=''> | | <StructureSection load='7ndh' size='340' side='right'caption='[[7ndh]], [[Resolution|resolution]] 1.94Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[7ndh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NDH FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7ndh]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NDH OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NDH FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.94Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Zc3h12c, Kiaa1726 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> |
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ndh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ndh OCA], [https://pdbe.org/7ndh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ndh RCSB], [https://www.ebi.ac.uk/pdbsum/7ndh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ndh ProSAT]</span></td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7ndh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7ndh OCA], [https://pdbe.org/7ndh PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7ndh RCSB], [https://www.ebi.ac.uk/pdbsum/7ndh PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7ndh ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[https://www.uniprot.org/uniprot/ZC12C_MOUSE ZC12C_MOUSE]] May function as RNase and regulate the levels of target RNA species.
| + | [https://www.uniprot.org/uniprot/ZC12C_MOUSE ZC12C_MOUSE] May function as RNase and regulate the levels of target RNA species. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </StructureSection> | | </StructureSection> |
| | [[Category: Large Structures]] | | [[Category: Large Structures]] |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Mus musculus]] |
| - | [[Category: Garg, A]] | + | [[Category: Garg A]] |
| - | [[Category: Heinemann, U]] | + | [[Category: Heinemann U]] |
| - | [[Category: Endo-ribonuclease]]
| + | |
| - | [[Category: Regnase]]
| + | |
| - | [[Category: Rna binding protein]]
| + | |
| Structural highlights
Function
ZC12C_MOUSE May function as RNase and regulate the levels of target RNA species.
Publication Abstract from PubMed
The CCCH-type zinc finger (ZnF) containing ZC3H12 ribonucleases are crucial in post-transcriptional immune homoeostasis with ZC3H12A being the only structurally studied member of the family. In this study, we present a structural-biochemical characterization of ZC3H12C, which is linked with chronic immune disorders like psoriasis. We established that the RNA substrate is cooperatively recognized by the PIN and ZnF domains of ZC3H12C and analyzed the crystal structure of ZC3H12C bound to a single-stranded RNA substrate. The RNA engages in hydrogen-bonded contacts and stacking interactions with the PIN and ZnF domains simultaneously. The ZC3H12 ZnF shows unprecedented structural features not previously observed in any member of the CCCH-ZnF family and utilizes stacking interactions via a unique combination of spatially conserved aromatic residues to align the target transcript in a bent conformation onto the ZnF scaffold. Further comparative structural analysis of ZC3H12 CCCH-ZnF suggests that a trinucleotide sequence is recognized by ZC3H12 ZnF in target RNA. Our work not only describes the initial structure-biochemical study on ZC3H12C, but also provides the first molecular insight into RNA recognition by a ZC3H12 family member. Finally, our work points to an evolutionary code for RNA recognition adopted by CCCH-type ZnF proteins.
PIN and CCCH Zn-finger domains coordinate RNA targeting in ZC3H12 family endoribonucleases.,Garg A, Roske Y, Yamada S, Uehata T, Takeuchi O, Heinemann U Nucleic Acids Res. 2021 May 5. pii: 6266460. doi: 10.1093/nar/gkab316. PMID:33950203[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Garg A, Roske Y, Yamada S, Uehata T, Takeuchi O, Heinemann U. PIN and CCCH Zn-finger domains coordinate RNA targeting in ZC3H12 family endoribonucleases. Nucleic Acids Res. 2021 May 5. pii: 6266460. doi: 10.1093/nar/gkab316. PMID:33950203 doi:http://dx.doi.org/10.1093/nar/gkab316
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