7nq4
From Proteopedia
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| - | ==== | + | ==Human tRNA guanine transglycosylase (TGT), RNA-bound covalent intermediate== |
| - | <StructureSection load='7nq4' size='340' side='right'caption='[[7nq4]]' scene=''> | + | <StructureSection load='7nq4' size='340' side='right'caption='[[7nq4]], [[Resolution|resolution]] 2.88Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[7nq4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=7NQ4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=7NQ4 FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nq4 OCA], [https://pdbe.org/7nq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nq4 RCSB], [https://www.ebi.ac.uk/pdbsum/7nq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nq4 ProSAT]</span></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.88Å</td></tr> |
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=9DG:9-DEAZAGUANINE'>9DG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=7nq4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=7nq4 OCA], [https://pdbe.org/7nq4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=7nq4 RCSB], [https://www.ebi.ac.uk/pdbsum/7nq4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=7nq4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | == Function == | ||
| + | [https://www.uniprot.org/uniprot/TGT_HUMAN TGT_HUMAN] Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product (By similarity).[UniProtKB:P28720][HAMAP-Rule:MF_03218]<ref>PMID:11255023</ref> <ref>PMID:20354154</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The eukaryotic tRNA guanine transglycosylase (TGT) is an RNA modifying enzyme incorporating queuine, a hypermodified guanine derivative, into the tRNAs(Asp,Asn,His,Tyr). While both subunits of the functional heterodimer have been crystallized individually, much of our understanding of its dimer interface or recognition of a target RNA has been inferred from its more thoroughly studied bacterial homolog. However, since bacterial TGT, by incorporating queuine precursor preQ(1), deviates not only in function, but as a homodimer, also in its subunit architecture, any inferences regarding the subunit association of the eukaryotic heterodimer or the significance of its unique catalytically inactive subunit are based on unstable footing. Here, we report the crystal structure of human TGT in its heterodimeric form and in complex with a 25-mer stem loop RNA, enabling detailed analysis of its dimer interface and interaction with a minimal substrate RNA. Based on a model of bound tRNA, we addressed a potential functional role of the catalytically inactive subunit QTRT2 by UV-crosslinking and mutagenesis experiments, identifying the two-stranded betaEbetaF-sheet of the QTRT2 subunit as an additional RNA-binding motif. | ||
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| + | Structural and functional insights into human tRNA guanine transgylcosylase.,Sievers K, Welp L, Urlaub H, Ficner R RNA Biol. 2021 Oct 15;18(sup1):382-396. doi: 10.1080/15476286.2021.1950980. Epub , 2021 Jul 31. PMID:34241577<ref>PMID:34241577</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 7nq4" style="background-color:#fffaf0;"></div> | ||
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| + | ==See Also== | ||
| + | *[[TRNA-guanine transglycosylase 3D structures|TRNA-guanine transglycosylase 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Homo sapiens]] | ||
[[Category: Large Structures]] | [[Category: Large Structures]] | ||
| - | [[Category: | + | [[Category: Synthetic construct]] |
| + | [[Category: Ficner R]] | ||
| + | [[Category: Sievers K]] | ||
Current revision
Human tRNA guanine transglycosylase (TGT), RNA-bound covalent intermediate
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